Back
Acetylation of intrinsically disordered regions regulates phase separation.
Journal article

Acetylation of intrinsically disordered regions regulates phase separation.

  • Saito M Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
  • Hess D Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
  • Eglinger J Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
  • Fritsch AW Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
  • Kreysing M Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
  • Weinert BT Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Choudhary C Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Matthias P Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland. patrick.matthias@fmi.ch.
Show more…
  • 2018-12-12
Published in:
  • Nature chemical biology. - 2019
Acetylation
Animals
Catalytic Domain
Cytoplasmic Granules
DEAD-box RNA Helicases
Gene Knockout Techniques
Histone Deacetylase 6
Humans
Intrinsically Disordered Proteins
Lysine
Mice
Models, Theoretical
Osmotic Pressure
RNA Helicases
English Liquid-liquid phase separation (LLPS) of proteins containing intrinsically disordered regions (IDRs) has been proposed as a mechanism underlying the formation of membrane-less organelles. Tight regulation of IDR behavior is essential to ensure that LLPS only takes place when necessary. Here, we report that IDR acetylation/deacetylation regulates LLPS and assembly of stress granules (SGs), membrane-less organelles forming in response to stress. Acetylome analysis revealed that the RNA helicase DDX3X, an important component of SGs, is a novel substrate of the deacetylase HDAC6. The N-terminal IDR of DDX3X (IDR1) can undergo LLPS in vitro, and its acetylation at multiple lysine residues impairs the formation of liquid droplets. We also demonstrated that enhanced LLPS propensity through deacetylation of DDX3X-IDR1 by HDAC6 is necessary for SG maturation, but not initiation. Our analysis provides a mechanistic framework to understand how acetylation and deacetylation of IDRs regulate LLPS spatiotemporally, and impact membrane-less organelle formation in vivo.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/270412
Statistics
Document views: 8 File downloads: