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A novel environment-evoked transcriptional signature predicts reactivity in single dentate granule neurons.
Journal article

A novel environment-evoked transcriptional signature predicts reactivity in single dentate granule neurons.

  • Jaeger BN The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Linker SB The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Parylak SL The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Barron JJ The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Gallina IS The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Saavedra CD The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Fitzpatrick C The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Lim CK The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Schafer ST The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Lacar B The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA.
  • Jessberger S Laboratory of Neural Plasticity, Faculty of Medicine and Science, Brain Research Institute, University of Zurich, 8057, Zurich, Switzerland.
  • Gage FH The Salk Institute for Biological Studies, La Jolla, CA, 92037-1002, USA. gage@salk.edu.
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  • 2018-08-08
Published in:
  • Nature communications. - 2018
Animals
CA1 Region, Hippocampal
Cytoplasmic Granules
Dentate Gyrus
Female
Gene Expression Profiling
Gene Expression Regulation
Interneurons
Memory
Mice
Mice, Inbred C57BL
Models, Neurological
Neurogenesis
Neuronal Plasticity
Neurons
Pyramidal Cells
Stochastic Processes
Time Factors
Transcription, Genetic
Vasoactive Intestinal Peptide
English Activity-induced remodeling of neuronal circuits is critical for memory formation. This process relies in part on transcription, but neither the rate of activity nor baseline transcription is equal across neuronal cell types. In this study, we isolated mouse hippocampal populations with different activity levels and used single nucleus RNA-seq to compare their transcriptional responses to activation. One hour after novel environment exposure, sparsely active dentate granule (DG) neurons had a much stronger transcriptional response compared to more highly active CA1 pyramidal cells and vasoactive intestinal polypeptide (VIP) interneurons. Activity continued to impact transcription in DG neurons up to 5 h, with increased heterogeneity. By re-exposing the mice to the same environment, we identified a unique transcriptional signature that selects DG neurons for reactivation upon re-exposure to the same environment. These results link transcriptional heterogeneity to functional heterogeneity and identify a transcriptional correlate of memory encoding in individual DG neurons.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/271416
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