Back
Full-text
Journal article

Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma.

  • Farrera-Soler L Department of Organic Chemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva, Geneva, Switzerland.
  • Daguer JP Department of Organic Chemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva, Geneva, Switzerland.
  • Barluenga S Department of Organic Chemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva, Geneva, Switzerland.
  • Vadas O Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland.
  • Cohen P Division of Laboratory Medicine, Diagnostic Department, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
  • Pagano S Division of Laboratory Medicine, Diagnostic Department, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
  • Yerly S Division of Laboratory Medicine, Diagnostic Department, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
  • Kaiser L Division of Laboratory Medicine, Diagnostic Department, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
  • Vuilleumier N Division of Laboratory Medicine, Diagnostic Department, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.
  • Winssinger N Department of Organic Chemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva, Geneva, Switzerland.
Show more…
  • 2020-09-09
Published in:
  • PloS one. - 2020
Amino Acid Sequence
Antibodies, Viral
Betacoronavirus
Coronavirus Infections
Epitope Mapping
Epitopes
Furin
Humans
Pandemics
Peptide Nucleic Acids
Peptides
Pneumonia, Viral
Protein Array Analysis
Protein Structure, Tertiary
Proteolysis
Recombinant Proteins
Sequence Alignment
Spike Glycoprotein, Coronavirus
English A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2') known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/272294
Statistics
Document views: 67 File downloads:
  • Full-text: 0