Blood culture-based diagnosis of bacteraemia: state of the art.
- Opota O Institute of Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.
- Croxatto A Institute of Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.
- Prod'hom G Institute of Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.
- Greub G Institute of Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland; Infectious Diseases Service, University of Lausanne and University Hospital Centre, Lausanne, Switzerland. Electronic address: Gilbert.Greub@chuv.ch.
- 2015-03-11
Published in:
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. - 2015
Bacteraemia
MALDI TOF MS
bacterial pellet
blood culture
bloodstream infection
diagnostic
Bacteremia
Bacteria
Bacteriological Techniques
Blood
Humans
Molecular Diagnostic Techniques
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
English
Blood culture remains the best approach to identify the incriminating microorganisms when a bloodstream infection is suspected, and to guarantee that the antimicrobial treatment is adequate. Major improvements have been made in the last years to increase the sensitivity and specificity and to reduce the time to identification of microorganisms recovered from blood cultures. Among other factors, the introduction in clinical microbiology laboratories of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry technology revolutionized the identification of microorganisms whereas the introduction of nucleic-acid-based methods, such as DNA hybridization or rapid PCR-based test, significantly reduce the time to results. Together with traditional antimicrobial susceptibility testing, new rapid methods for the detection of resistance mechanisms respond to major epidemiological concerns such as methicillin-resistant Staphylococcus aureus, extended-spectrum β-lactamase or carbapenemases. This review presents and discusses the recent developments in microbial diagnosis of bloodstream infections based on blood cultures.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1016/j.cmi.2015.01.003
- PMID 25753137
- Persistent URL
- https://sonar.ch/global/documents/27366
Statistics
Document views: 52
File downloads:
- Full-text: 0