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Pantoea gaviniae sp. nov. and Pantoea calida sp. nov., isolated from infant formula and an infant formula production environment.

  • Popp A Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland.
  • Cleenwerck I BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, 9000 Gent, Belgium.
  • Iversen C Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
  • De Vos P BCCM/LMG Bacteria Collection, Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, 9000 Gent, Belgium.
  • Stephan R Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland.
  • 2010-01-12
Published in:
  • International journal of systematic and evolutionary microbiology. - 2010
Bacterial Typing Techniques
DNA, Bacterial
Food Contamination
Food-Processing Industry
Genes, Bacterial
Infant Formula
Molecular Sequence Data
Multilocus Sequence Typing
Nucleic Acid Hybridization
Pantoea
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
English Five Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped bacterial isolates were obtained from infant formula and an infant formula production environment and were investigated by use of a polyphasic taxonomic study. Biochemical tests and partial rpoB gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family Enterobacteriaceae, closely related to several species of the genera Pantoea and Erwinia, which indicated a phylogenetic position within the genus Pantoea or the genus Erwinia. Multilocus sequence analysis of concatenated partial atpD, gyrB, infB and rpoB gene sequences of two of the isolates suggested that they represented two novel species of the genus Pantoea, phylogenetically related most closely to Pantoea septica. The five isolates had general characteristics consistent with those of the genus Pantoea, and DNA-DNA hybridizations between two representatives and the type strains of their phylogenetically closest relatives based on comparative 16S rRNA gene sequence analysis showed that the isolates represented two novel genospecies. These two genospecies could be differentiated from each other based on fermentation of galacturonate, sorbitol and potassium 5-ketogluconate. They could be differentiated from phylogenetically related Pantoea species based on their ability to ferment lactose and to utilize β-gentiobiose and raffinose, their inability to ferment or utilize d-arabitol, and their inability to produce indole. On the basis of the results obtained, the five isolates are considered to represent two novel species of the genus Pantoea, for which the names Pantoea gaviniae sp. nov. (type strain A18/07(T) =LMG 25382(T) =DSM 22758(T)) and Pantoea calida sp. nov. (type strain 1400/07(T) =LMG 25383(T) =DSM 22759(T)) are proposed.
Language
  • English
Open access status
bronze
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https://sonar.ch/global/documents/27484
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