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Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo.
Journal article

Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo.

  • Zhu Z Southeast University, Key Laboratory of MEMS of Ministry of Education, Nanjing, China. zhuzhen@seu.edu.cn.
  • Frey O Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
  • Hierlemann A Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
  • 2018-02-10
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2018
Cell cycle
Cell growth
Electrical impedance spectroscopy
Microfluidics
Nuclear division
Schizosaccharomyces pombe
Dielectric Spectroscopy
G2 Phase
Mitosis
Schizosaccharomyces
English This chapter describes a microfluidic device that enables immobilization and culturing of single rod-shaped S. pombe cells in a stand-up mode. The wide-band electrical impedance spectroscopy (EIS) has been integrated in the microfluidic device to continuously measure cell growth of single S. pombe cells. Cell growth curves showing cellular and intracellular features at high spatiotemporal resolution can be obtained from EIS signals. The features include longitudinal cell elongation in the G2 phase, mitosis, and cell division during an entire cell cycle of S. pombe cells. Microfluidics-based EIS systems provide, hence, a tool for dynamic single-cell studies.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/277317
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