Back
Full-text
Journal article

Molecular Basis of Bacterial Host Interactions by Gram-Positive Targeting Bacteriophages.

  • Dunne M Institute of Food Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland. mdunne@ethz.ch.
  • Hupfeld M Institute of Food Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland. hupfeldm@protonmail.ch.
  • Klumpp J Institute of Food Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland. jochen.klumpp@hest.ethz.ch.
  • Loessner MJ Institute of Food Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland. martin.loessner@ethz.ch.
Show more…
  • 2018-08-01
Published in:
  • Viruses. - 2018
Bacillus subtilis
Lactococcus lactis
Listeria monocytogenes
Staphylococcus aureus
bacteriophage
gram-positive bacteria
infection
phage technology
receptor-binding proteins
Bacillus subtilis
Bacteriophages
Crystallography, X-Ray
Gram-Positive Bacteria
Host Specificity
Lactococcus lactis
Listeria monocytogenes
Microscopy, Electron
Models, Molecular
Protein Binding
Siphoviridae
Staphylococcus Phages
Staphylococcus aureus
English The inherent ability of bacteriophages (phages) to infect specific bacterial hosts makes them ideal candidates to develop into antimicrobial agents for pathogen-specific remediation in food processing, biotechnology, and medicine (e.g., phage therapy). Conversely, phage contaminations of fermentation processes are a major concern to dairy and bioprocessing industries. The first stage of any successful phage infection is adsorption to a bacterial host cell, mediated by receptor-binding proteins (RBPs). As the first point of contact, the binding specificity of phage RBPs is the primary determinant of bacterial host range, and thus defines the remediative potential of a phage for a given bacterium. Co-evolution of RBPs and their bacterial receptors has forced endless adaptation cycles of phage-host interactions, which in turn has created a diverse array of phage adsorption mechanisms utilizing an assortment of RBPs. Over the last decade, these intricate mechanisms have been studied intensely using electron microscopy and X-ray crystallography, providing atomic-level details of this fundamental stage in the phage infection cycle. This review summarizes current knowledge surrounding the molecular basis of host interaction for various socioeconomically important Gram-positive targeting phage RBPs to their protein- and saccharide-based receptors. Special attention is paid to the abundant and best-characterized Siphoviridae family of tailed phages. Unravelling these complex phage-host dynamics is essential to harness the full potential of phage-based technologies, or for generating novel strategies to combat industrial phage contaminations.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/277612
Statistics
Document views: 12 File downloads:
  • fulltext.pdf: 0