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Typing of Listeria monocytogenes by monocin and phage receptors.
Journal article

Typing of Listeria monocytogenes by monocin and phage receptors.

  • Bannerman E Centre National de Référence des Listérias, WHO Collaborating Center for Foodborne Listeriosis, CHUV, Lausanne, Switzerland.
  • Boerlin P
  • Bille J
  • 1996-08-01
Published in:
  • International journal of food microbiology. - 1996
Bacterial Typing Techniques
Bacteriocins
Bacteriophage Typing
Listeria monocytogenes
Receptors, Virus
Reproducibility of Results
English One hundred strains of Listeria monocytogenes from both sporadic and epidemic cases were typed by monocin production combined with phage receptor and reverse phage receptor methods. The monocin-phage combination gave 72 types with 100% typability and 97% reproducibility. The results were compared to those of serotyping, phage typing, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and RAPD (random amplification of polymorphic DNA). The monocin/phage types were comparable in terms of discrimination with other methods for epidemiological investigations. The index of discrimination of using the monocin typing and phage receptor/reverse phage receptor method combination (0.99) for both the 87 epidemiologically unrelated strains and the epidemiologically important serogroup 4 strains was the highest of the seven different methods analysed. This combination of methods was simple, highly discriminatory and reproducible and can be carried out in a non-specialized laboratory. However, like most of the other Listeria typing methods, both the method and the indicator test strains need to be standardized.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/277682
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