Typing of Listeria monocytogenes by monocin and phage receptors.

Bannerman E; Boerlin P; Bille J

doi:10.1016/0168-1605(96)01003-3

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Journal article

Typing of Listeria monocytogenes by monocin and phage receptors.

Bannerman E Centre National de Référence des Listérias, WHO Collaborating Center for Foodborne Listeriosis, CHUV, Lausanne, Switzerland.
Boerlin P
Bille J

1996-08-01

Published in:

International journal of food microbiology. - 1996

English One hundred strains of Listeria monocytogenes from both sporadic and epidemic cases were typed by monocin production combined with phage receptor and reverse phage receptor methods. The monocin-phage combination gave 72 types with 100% typability and 97% reproducibility. The results were compared to those of serotyping, phage typing, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and RAPD (random amplification of polymorphic DNA). The monocin/phage types were comparable in terms of discrimination with other methods for epidemiological investigations. The index of discrimination of using the monocin typing and phage receptor/reverse phage receptor method combination (0.99) for both the 87 epidemiologically unrelated strains and the epidemiologically important serogroup 4 strains was the highest of the seven different methods analysed. This combination of methods was simple, highly discriminatory and reproducible and can be carried out in a non-specialized laboratory. However, like most of the other Listeria typing methods, both the method and the indicator test strains need to be standardized.

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