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Insulin inhibits tyrosinase activity and 5-S-cysteinyldopa formation in human melanoma cells.
Journal article

Insulin inhibits tyrosinase activity and 5-S-cysteinyldopa formation in human melanoma cells.

  • Benathan M Department of Dermatology, Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland.
  • Labidi F
  • 1997-07-01
Published in:
  • Acta dermato-venereologica. - 1997
Calcium
Chromatography, High Pressure Liquid
Cysteinyldopa
Glutathione Peroxidase
Glutathione Reductase
Humans
Insulin
Melanins
Melanoma
Monophenol Monooxygenase
Skin Neoplasms
Sodium Selenite
Tumor Cells, Cultured
English The effects of insulin on melanogenesis were examined in human Swift melanoma cells. When these cells were grown in a chemically defined culture medium containing insulin (5 microg/ml), they showed a low pigmentation in association with a high activity of glutathione peroxidase (GPO) and a low activity of tyrosinase. In Eagle's minimum essential medium supplemented with foetal calf serum (EMEM-FCS), the Swift cells showed an intense pigmentation in association with a low GPO activity and a high tyrosinase activity. Modulation of GPO activity with sodium selenite had no effect on melanogenesis variables. In contrast, addition of insulin (5 microg/ml) to the EMEM medium led to a marked decrease in tyrosinase activity (p<0.001) and to a concomitant reduction in the levels of 5-S-cysteinyldopa (p <0.01). These results indicate that insulin inhibits the formation of 5-S-cysteinyldopa and that of melanin via the inhibition of tyrosinase activity.
Language
  • English
Identifiers
Persistent URL
https://sonar.ch/global/documents/277712
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