Journal article
A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.
- Peitsch MC Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
- Kovacsovics TJ
- Isliker H
- 1988-04-06
Published in:
- Journal of immunological methods. - 1988
Chromatography, High Pressure Liquid
Complement Activating Enzymes
Complement C1
Complement C1r
Complement C1s
Complement Pathway, Classical
Enzyme Activation
Enzyme Precursors
Humans
English
The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/0022-1759(88)90428-0
- PMID 2832479
- Persistent URL
- https://sonar.ch/global/documents/277736
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