Back
Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.
Journal article

Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.

  • Gerganova V Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
  • Floderer C Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Archetti A Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Michon L Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
  • Carlini L Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Reichler T Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
  • Manley S Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Martin SG Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
Show more…
  • 2019-05-04
Published in:
  • eLife. - 2019
Pom1 DYRK kinase
S. pombe
cell biology
cell size
concentration gradient
diffusion
single particle tracking PALM
Cell Membrane
Cytoplasm
Phosphorylation
Protein Binding
Protein Kinases
Protein Processing, Post-Translational
Protein-Serine-Threonine Kinases
Schizosaccharomyces
Schizosaccharomyces pombe Proteins
English Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rely on membrane association regulated by a phosphorylation-dephosphorylation cycle and lateral diffusion modulated by clustering. Using quantitative PALM imaging, we find individual Pom1 molecules bind the membrane too transiently to diffuse from pole to mid-cell. Instead, we propose they exchange within longer lived clusters forming the functional gradient unit. An allelic series blocking auto-phosphorylation shows that multi-phosphorylation shapes and buffers the gradient to control mid-cell levels, which represent the critical Cdr2-regulating pool. TIRF imaging of this cortical pool demonstrates more Pom1 overlaps with Cdr2 in short than long cells, consistent with Pom1 inhibition of Cdr2 decreasing with cell growth. Thus, the gradients modulate Pom1 mid-cell levels according to cell size.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/277994
Statistics
Document views: 37 File downloads: