Journal article
Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape (Brassica napus).
- Coleto I Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain.
- de la Peña M Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain.
- Rodríguez-Escalante J Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain.
- Bejarano I Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain.
- Glauser G Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, Avenue de Bellevaux 51, 2000, Neuchâtel, Switzerland.
- Aparicio-Tejo PM Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Navarre, Spain.
- González-Moro MB Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain.
- Marino D Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain. daniel.marino@ehu.eus.
- 2017-09-22
Published in:
- BMC plant biology. - 2017
Ammonium
Brassica napus
Glucosinolates
Nitrate
Nitrogen
Oilseed rape
Sulfur
Acclimatization
Ammonium Compounds
Brassica napus
Nitrogen
Plant Leaves
Plant Proteins
Sulfur
English
BACKGROUND
The coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioning of S metabolism. The aim of this work was to provide an overview of N and S metabolism in oilseed rape (Brassica napus) when exposed to different N sources. To do so, plants were grown in hydroponic conditions with nitrate or ammonium as N source at two concentrations (0.5 and 1 mM).
RESULTS
Metabolic changes mainly occurred in leaves, where ammonium caused the up-regulation of enzymes involved in the primary assimilation of N and a general increase in the concentration of N-compounds (NH4+, amino acids and proteins). Similarly, the activity of key enzymes of primary S assimilation and the content of S-compounds (glutathione and glucosinolates) were also higher in leaves of ammonium-fed plants. Interestingly, sulfate level was lower in leaves of ammonium-fed plants, which was accompanied by the down-regulation of SULTR1 transporters gene expression.
CONCLUSIONS
The results highlight the impact of the N source on different steps of N and S metabolism in oilseed rape, notably inducing N and S assimilation in leaves, and put forward the potential of N source management to modulate the synthesis of compounds with biotechnological interest, such as glucosinolates.
The coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioning of S metabolism. The aim of this work was to provide an overview of N and S metabolism in oilseed rape (Brassica napus) when exposed to different N sources. To do so, plants were grown in hydroponic conditions with nitrate or ammonium as N source at two concentrations (0.5 and 1 mM).
RESULTS
Metabolic changes mainly occurred in leaves, where ammonium caused the up-regulation of enzymes involved in the primary assimilation of N and a general increase in the concentration of N-compounds (NH4+, amino acids and proteins). Similarly, the activity of key enzymes of primary S assimilation and the content of S-compounds (glutathione and glucosinolates) were also higher in leaves of ammonium-fed plants. Interestingly, sulfate level was lower in leaves of ammonium-fed plants, which was accompanied by the down-regulation of SULTR1 transporters gene expression.
CONCLUSIONS
The results highlight the impact of the N source on different steps of N and S metabolism in oilseed rape, notably inducing N and S assimilation in leaves, and put forward the potential of N source management to modulate the synthesis of compounds with biotechnological interest, such as glucosinolates.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1186/s12870-017-1100-9
- PMID 28931380
- Persistent URL
- https://sonar.ch/global/documents/278140
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