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Durable and controlled depletion of neutrophils in mice.
Journal article

Durable and controlled depletion of neutrophils in mice.

  • Boivin G Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Faget J Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Ancey PB Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Gkasti A Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Mussard J University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon, 69373, Lyon, France.
  • Engblom C Center for Systems Biology, Massachusetts General Hospital Research Institute and Harvard Medical School, Boston, MA, 02114, USA.
  • Pfirschke C Center for Systems Biology, Massachusetts General Hospital Research Institute and Harvard Medical School, Boston, MA, 02114, USA.
  • Contat C Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Pascual J Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Vazquez J Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
  • Bendriss-Vermare N University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon, 69373, Lyon, France.
  • Caux C University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon, 69373, Lyon, France.
  • Vozenin MC Laboratory of Radiation Oncology, Department of Radiation Oncology, Department of Oncology, CHUV, Lausanne University Hospital and University of Lausanne, CH-1011, Lausanne, Switzerland.
  • Pittet MJ Center for Systems Biology, Massachusetts General Hospital Research Institute and Harvard Medical School, Boston, MA, 02114, USA.
  • Gunzer M Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
  • Meylan E Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland. etienne.meylan@epfl.ch.
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  • 2020-06-04
Published in:
  • Nature communications. - 2020
Animals
Antibodies, Monoclonal
Antigens, Ly
Bone Marrow
Cell Death
Disease Models, Animal
Gene Expression
Immunity, Innate
Mice
Mice, Inbred C57BL
Neutrophils
RNA, Messenger
English Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/278275
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