Journal article
Differential influence of maternal and fetal pregnancy factors on the in-vitro induction of human regulatory T cells: a preliminary study.
- Marcoli N Department of Rheumatology, Immunology and Allergology, University Hospital and University of Bern, Switzerland.
- Østensen M Department of Rheumatology, Immunology and Allergology, University Hospital and University of Bern, Switzerland.
- Portmann-Lanz CB Department of Obstetrics and Gynaecology and Department of Clinical Research, University Hospital and University of Bern, Switzerland.
- Surbek D Department of Obstetrics and Gynaecology and Department of Clinical Research, University Hospital and University of Bern, Switzerland.
- Villiger PM Department of Rheumatology, Immunology and Allergology, University Hospital and University of Bern, Switzerland.
- Förger F Department of Rheumatology, Immunology and Allergology, University Hospital and University of Bern, Switzerland.
- 2015-08-01
Published in:
- Swiss medical weekly. - 2015
Adult
CD4-Positive T-Lymphocytes
Cells, Cultured
Chorionic Villi
Cross-Sectional Studies
Cytokines
Female
Humans
Interleukin-2 Receptor alpha Subunit
Leukocytes, Mononuclear
Middle Aged
Pregnancy
Pregnancy Trimester, Second
Pregnancy Trimester, Third
T-Lymphocytes, Regulatory
English
PROBLEM
Given the important role of regulatory T cells (Treg) for successful pregnancy, the ability of soluble maternal and fetal pregnancy factors to induce human Treg was investigated.
METHOD OF STUDY
Peripheral blood mononuclear cells (PBMCs) or isolated CD4+CD25‒ cells were cultured in the presence of pooled second or third trimester pregnancy sera, steroid hormones or supernatants from placental explants, and the numbers and function of induced CD4+CD25+FOXP3+ Treg were analysed.
RESULTS
Third trimester pregnancy sera and supernatants of early placental explants, but not sex steroid hormones, induced an increase of Tregs from PBMCs. Early placental supernatant containing high levels of tumour necrosis factor-α, interferon-γ, interleukins -1, -6 and -17, soluble human leucocyte antigen-G, and transforming growth factor-β1, increased the proportion of Treg most effectively and was able to induce interleukin-10-secreting-Treg from CD4+CD25‒cells.
CONCLUSIONS
Compared with circulating maternal factors, placental- and fetal-derived factors appear to exert a more powerful effect on numerical changes of Treg, thereby supporting fetomaternal tolerance during human pregnancy.
Given the important role of regulatory T cells (Treg) for successful pregnancy, the ability of soluble maternal and fetal pregnancy factors to induce human Treg was investigated.
METHOD OF STUDY
Peripheral blood mononuclear cells (PBMCs) or isolated CD4+CD25‒ cells were cultured in the presence of pooled second or third trimester pregnancy sera, steroid hormones or supernatants from placental explants, and the numbers and function of induced CD4+CD25+FOXP3+ Treg were analysed.
RESULTS
Third trimester pregnancy sera and supernatants of early placental explants, but not sex steroid hormones, induced an increase of Tregs from PBMCs. Early placental supernatant containing high levels of tumour necrosis factor-α, interferon-γ, interleukins -1, -6 and -17, soluble human leucocyte antigen-G, and transforming growth factor-β1, increased the proportion of Treg most effectively and was able to induce interleukin-10-secreting-Treg from CD4+CD25‒cells.
CONCLUSIONS
Compared with circulating maternal factors, placental- and fetal-derived factors appear to exert a more powerful effect on numerical changes of Treg, thereby supporting fetomaternal tolerance during human pregnancy.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.4414/smw.2015.14172
- PMID 26230352
- Persistent URL
- https://sonar.ch/global/documents/278576
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