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Automated computer-assisted quantitative analysis of intact murine lungs at the alveolar scale.

  • Lovric G Centre d'Imagerie BioMédicale, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Vogiatzis Oikonomidis I Swiss Light Source, Paul Scherrer Institute, 5234 Villigen, Switzerland.
  • Mokso R Max IV Laboratory, Lund University, SE-221 00 Lund, Sweden.
  • Stampanoni M Swiss Light Source, Paul Scherrer Institute, 5234 Villigen, Switzerland.
  • Roth-Kleiner M Clinic of Neonatology, University Hospital of Lausanne (CHUV), 1011 Lausanne, Switzerland.
  • Schittny JC Institute of Anatomy, University of Bern, 3012 Bern, Switzerland.
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  • 2017-09-22
Published in:
  • PloS one. - 2017
Animals
Automation
Imaging, Three-Dimensional
Mice
Microscopy
Pulmonary Alveoli
Tomography, X-Ray Computed
English Using state-of-the-art X-ray tomographic microscopy we can image lung tissue in three dimensions in intact animals down to a micrometer precision. The structural complexity and hierarchical branching scheme of the lung at this level of details, however, renders the extraction of biologically relevant quantities particularly challenging. We have developed a methodology for a detailed description of lung inflation patterns by measuring the size and the local curvature of the parenchymal airspaces. These quantitative tools for morphological and topological analyses were applied to high-resolution murine 3D lung image data, inflated at different pressure levels under immediate post mortem conditions. We show for the first time direct indications of heterogeneous intra-lobar and inter-lobar distension patterns at the alveolar level. Furthermore, we did not find any indication that a cyclic opening-and-collapse (recruitment) of a large number of alveoli takes place.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/278607
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