On the micro-indentation of plant cells in a tissue context.
- Mosca G Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany. Institute of Plant Sciences, University of Bern, Bern, Switzerland.
- Sapala A
- Strauss S
- Routier-Kierzkowska AL
- Smith RS
- 2017-02-10
Published in:
- Physical biology. - 2017
Arabidopsis
Biomechanical Phenomena
Computer Simulation
Elasticity
Finite Element Analysis
Models, Biological
Osmotic Pressure
Single-Cell Analysis
English
The effect of geometry on cell stiffness measured with micro-indentation techniques has been explored in single cells, however it is unclear if results on single cells can be readily transferred to indentation experiments performed on a tissue in vivo. Here we explored this question by using simulation models of osmotic treatments and micro-indentation experiments on 3D multicellular tissues with the finite element method. We found that the cellular context does affect measured cell stiffness, and that several cells of context in each direction are required for optimal results. We applied the model to micro-indentation data obtained with cellular force microscopy on the sepal of A. thaliana, and found that differences in measured stiffness could be explained by cellular geometry, and do not necessarily indicate differences in cell wall material properties or turgor pressure.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1088/1478-3975/aa5698
- PMID 28181475
- Persistent URL
- https://sonar.ch/global/documents/278691
Statistics
Document views: 25
File downloads:
- Full-text: 0