Journal article
Perfusion-decellularization of human ear grafts enables ECM-based scaffolds for auricular vascularized composite tissue engineering.
- Duisit J Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium; Department of Plastic and Reconstructive Surgery, Cliniques Universitaires Saint-Luc, Avenue Hippocrate 10, B-1200 Brussels, Belgium. Electronic address: jerome.duisit@uclouvain.be.
- Amiel H Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium. Electronic address: hadrien.amiel@student.uclouvain.be.
- Wüthrich T Department for BioMedical Research, University of Bern, Murtenstrasse 50, CH-3008 Bern, Switzerland. Electronic address: tsering.w@students.unibe.ch.
- Taddeo A Department for BioMedical Research, University of Bern, Murtenstrasse 50, CH-3008 Bern, Switzerland; Department of Plastic, Reconstructive and Hand Surgery, Inselspital, University Hospital, CH-3010 Bern, Switzerland. Electronic address: adriano.taddeo@dbmr.unibe.ch.
- Dedriche A Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium. Electronic address: adeline.dedriche@uclouvain.be.
- Destoop V Institute of Mechanics, Materials and Civil Engineering, Materials and Process Engineering, Place Sainte Barbe 2/L5.02.02, B-1348 Louvain-la-Neuve, Belgium. Electronic address: vincent.destoop@uclouvain.be.
- Pardoen T Institute of Mechanics, Materials and Civil Engineering, Materials and Process Engineering, Place Sainte Barbe 2/L5.02.02, B-1348 Louvain-la-Neuve, Belgium. Electronic address: thomas.pardoen@uclouvain.be.
- Bouzin C Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium. Electronic address: caroline.bouzin@uclouvain.be.
- Joris V Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium. Electronic address: virginie.joris@uclouvain.be.
- Magee D School of Computing, University of Leeds, Leeds LS2 9JT, UK; HeteroGenius Limited, 21 Parkland Crescent, LS6 4PR Leeds, UK. Electronic address: D.R.Magee@leeds.ac.uk.
- Vögelin E Department of Plastic, Reconstructive and Hand Surgery, Inselspital, University Hospital, CH-3010 Bern, Switzerland. Electronic address: esther.voegelin@insel.ch.
- Harriman D Department of Surgery, Section of Transplantation, Wake Forest School of Medicine, 1 Medical Center Blvd, Winston-Salem, NC 27157, USA.
- Dessy C Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium. Electronic address: chantal.dessy@uclouvain.be.
- Orlando G Department of Surgery, Section of Transplantation, Wake Forest School of Medicine, 1 Medical Center Blvd, Winston-Salem, NC 27157, USA. Electronic address: gorlando@wakehealth.edu.
- Behets C Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium. Electronic address: catherine.behets@uclouvain.be.
- Rieben R Department for BioMedical Research, University of Bern, Murtenstrasse 50, CH-3008 Bern, Switzerland. Electronic address: robert.rieben@dbmr.unibe.ch.
- Gianello P Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium. Electronic address: pierre.gianello@uclouvain.be.
- Lengelé B Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain, Avenue Hippocrate 55/B1.55.04, B-1200 Brussels, Belgium; Department of Plastic and Reconstructive Surgery, Cliniques Universitaires Saint-Luc, Avenue Hippocrate 10, B-1200 Brussels, Belgium. Electronic address: benoit.lengele@uclouvain.be.
- 2018-04-15
Published in:
- Acta biomaterialia. - 2018
Ear graft
Extracellular matrix
Human
Perfusion-decellularization
Vascularized composite tissue engineering
Adipocytes
Animals
Biocompatible Materials
Bioreactors
Blood Pressure
Cadaver
DNA
Ear
Extracellular Matrix
Fluoroscopy
Humans
Leukocytes, Mononuclear
Perfusion
Rats
Stem Cells
Stress, Mechanical
Swine
Tissue Engineering
Tissue Scaffolds
Tissue Transplantation
English
INTRODUCTION
Human ear reconstruction is recognized as the emblematic enterprise in tissue engineering. Up to now, it has failed to reach human applications requiring appropriate tissue complexity along with an accessible vascular tree. We hereby propose a new method to process human auricles in order to provide a poorly immunogenic, complex and vascularized ear graft scaffold.
METHODS
12 human ears with their vascular pedicles were procured. Perfusion-decellularization was applied using a SDS/polar solvent protocol. Cell and antigen removal was examined by histology and DNA was quantified. Preservation of the extracellular matrix (ECM) was assessed by conventional and 3D-histology, proteins and cytokines quantifications. Biocompatibility was assessed by implantation in rats for up to 60 days. Adipose-derived stem cells seeding was conducted on scaffold samples and with human aortic endothelial cells whole graft seeding in a perfusion-bioreactor.
RESULTS
Histology confirmed cell and antigen clearance. DNA reduction was 97.3%. ECM structure and composition were preserved. Implanted scaffolds were tolerated in vivo, with acceptable inflammation, remodeling, and anti-donor antibody formation. Seeding experiments demonstrated cell engraftment and viability.
CONCLUSIONS
Vascularized and complex auricular scaffolds can be obtained from human source to provide a platform for further functional auricular tissue engineered constructs, hence providing an ideal road to the vascularized composite tissue engineering approach.
STATEMENT OF SIGNIFICANCE
The ear is emblematic in the biofabrication of tissues and organs. Current regenerative medicine strategies, with matrix from donor tissues or 3D-printed, didn't reach any application for reconstruction, because critically missing a vascular tree for perfusion and transplantation. We previously described the production of vascularized and cell-compatible scaffolds, from porcine ear grafts. In this study, we ---- applied findings directly to human auricles harvested from postmortem donors, providing a perfusable matrix that retains the ear's original complexity and hosts new viable cells after seeding. This approach unlocks the ability to achieve an auricular tissue engineering approach, associated with possible clinical translation.
Human ear reconstruction is recognized as the emblematic enterprise in tissue engineering. Up to now, it has failed to reach human applications requiring appropriate tissue complexity along with an accessible vascular tree. We hereby propose a new method to process human auricles in order to provide a poorly immunogenic, complex and vascularized ear graft scaffold.
METHODS
12 human ears with their vascular pedicles were procured. Perfusion-decellularization was applied using a SDS/polar solvent protocol. Cell and antigen removal was examined by histology and DNA was quantified. Preservation of the extracellular matrix (ECM) was assessed by conventional and 3D-histology, proteins and cytokines quantifications. Biocompatibility was assessed by implantation in rats for up to 60 days. Adipose-derived stem cells seeding was conducted on scaffold samples and with human aortic endothelial cells whole graft seeding in a perfusion-bioreactor.
RESULTS
Histology confirmed cell and antigen clearance. DNA reduction was 97.3%. ECM structure and composition were preserved. Implanted scaffolds were tolerated in vivo, with acceptable inflammation, remodeling, and anti-donor antibody formation. Seeding experiments demonstrated cell engraftment and viability.
CONCLUSIONS
Vascularized and complex auricular scaffolds can be obtained from human source to provide a platform for further functional auricular tissue engineered constructs, hence providing an ideal road to the vascularized composite tissue engineering approach.
STATEMENT OF SIGNIFICANCE
The ear is emblematic in the biofabrication of tissues and organs. Current regenerative medicine strategies, with matrix from donor tissues or 3D-printed, didn't reach any application for reconstruction, because critically missing a vascular tree for perfusion and transplantation. We previously described the production of vascularized and cell-compatible scaffolds, from porcine ear grafts. In this study, we ---- applied findings directly to human auricles harvested from postmortem donors, providing a perfusable matrix that retains the ear's original complexity and hosts new viable cells after seeding. This approach unlocks the ability to achieve an auricular tissue engineering approach, associated with possible clinical translation.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1016/j.actbio.2018.04.009
- PMID 29654989
- Persistent URL
- https://sonar.ch/global/documents/278756
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