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Clonality testing as complementary tool in the assessment of different patient groups with canine chronic enteropathy.
Journal article

Clonality testing as complementary tool in the assessment of different patient groups with canine chronic enteropathy.

  • Luckschander-Zeller N Clinic for Internal Medicine, Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Austria. Electronic address: Nicole.Luckschander@vetmeduni.ac.at.
  • Hammer SE Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
  • Ruetgen BC Clinical Pathology Platform, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
  • Tichy A Bioinformatics and Biostatistics Platform, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria.
  • Thalhammer JG Clinic for Internal Medicine, Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Austria.
  • Haas E Clinic for Internal Medicine, Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Austria.
  • Richter B Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
  • Welle M Institute for Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Burgener IA Clinic for Internal Medicine, Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Austria.
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  • 2019-08-06
Published in:
  • Veterinary immunology and immunopathology. - 2019
Antigen receptor gene rearrangement
Canine chronic enteropathy
Canine intestinal lymphoma
Clonality testing
Unspecific amplification
Animals
Biopsy
Case-Control Studies
Chronic Disease
Diagnosis, Differential
Dog Diseases
Dogs
Female
Gene Rearrangement, T-Lymphocyte
Inflammatory Bowel Diseases
Intestines
Lymphoma
Male
Protein-Losing Enteropathies
Receptors, Antigen, T-Cell, gamma-delta
Retrospective Studies
English Differentiation between canine chronic enteropathy (CCE) and intestinal lymphoma is a diagnostic challenge as histopathology might fail to yield unequivocal results. Detection of clonal rearrangements of the T-cell-receptor gamma (TCRG) chain and IG heavy chain (IGH) V-J genes offer a useful solution. In this retrospective study, histopathology samples of 35 CCE patients and 7 healthy Beagle dogs underwent clonality testing. Patients suffered either from inflammatory bowel disease (IBD), food responsive diarrhea (FRD) or protein loosing enteropathy secondary to IBD (PLE/IBD). Healthy Beagles served as controls (CO). Canine IBD activity index (CIBDAI) and histopathological WSAVA-grading differed significantly (p<0.001) between groups. CIBDAI improved significantly after appropriate therapy (p < 0.0001). Intestinal biopsies of all CO showed polyclonal patterns for B- and T-cell primers. All samples from CCE patients showed polyclonal patterns for the B-cell primers. Targeting TCRG, 4 patients showed a monoclonal or oligoclonal pattern of the lymphocytic infiltrates in the duodenum and/or colon. Clinical improvement was observed in all dogs. Although a small cell lymphoma cannot be excluded in view of the short follow up duration, a false positive result, in the sense of a canonical rearrangement or unspecific amplification due to a antigenic stimulation in a non-neoplastic inflammatory process is possible.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/278890
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