Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey.
- Çağlayan K Plant Protection Department, Agriculture Faculty, Mustafa Kemal University, 31034 Hatay, Turkey. caglayan@mku.edu.tr.
- Roumi V Plant Protection Department, Faculty of Agriculture, University of Maragheh, 55181 Maragheh, Iran. vroumi@maragheh.ac.ir.
- Gazel M Plant Protection Department, Agriculture Faculty, Mustafa Kemal University, 31034 Hatay, Turkey. monagazel@hotmail.com.
- Elçi E Plant Production and Technologies Department, Faculty of Agricultural Sciences and Technologies, Nigde Omer Halisdemir University, 51240 Nigde, Turkey. eminur@gmail.com.
- Acioğlu M Plant Protection Department, Agriculture Faculty, Mustafa Kemal University, 31034 Hatay, Turkey. mehtappathem@hotmail.com.
- Mavric Plesko I Agricultural Institute of Slovenia, Hacquetova 17, SI- 1000 Ljubljana, Slovenia. irena.mavricplesko@kis.si.
- Reynard JS Virology-Phytoplasmology Laboratory, Agroscope, 1260 Nyon, Switzerland. jean-sebastien.reynard@agroscope.admin.ch.
- Maclot F Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Liege, Passage des Deportes, 2, 5030 Gembloux, Belgium. francois.maclot@doct.ulg.ac.be.
- Massart S Plant Pathology Laboratory, TERRA-Gembloux Agro-Bio Tech, University of Liege, Passage des Deportes, 2, 5030 Gembloux, Belgium. sebastien.massart@uliege.be.
- 2019-05-01
Published in:
- Pathogens (Basel, Switzerland). - 2019
English
High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5' UTR and 3' UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43-53%, 44-60%, 39-43%, 38-44% and 45-50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.3390/pathogens8020057
- PMID 31035571
- Persistent URL
- https://sonar.ch/global/documents/279111
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