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Characterisation of the mating-type locus in the genus Xanthoria (lichen-forming ascomycetes, Lecanoromycetes).
Journal article

Characterisation of the mating-type locus in the genus Xanthoria (lichen-forming ascomycetes, Lecanoromycetes).

  • 2005-11-04
Published in:
  • Fungal genetics and biology : FG & B. - 2005
Amino Acid Sequence
Ascomycota
Carrier Proteins
Cloning, Molecular
Cytoskeletal Proteins
DNA, Fungal
DNA-(Apurinic or Apyrimidinic Site) Lyase
Fungal Proteins
Gene Library
Gene Order
Genes, Fungal
Genes, Mating Type, Fungal
Molecular Sequence Data
Polymerase Chain Reaction
Saccharomyces cerevisiae Proteins
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Spores, Fungal
English Conserved regions of mating-type genes were amplified in four representatives of the genus Xanthoria (X. parietina, X. polycarpa, X. flammea, and X. elegans) using PCR-based methods. The complete MAT locus, containing one ORF (MAT1-2-1) coding for a truncated HMG-box protein, and two partial flanking genes, were cloned by screening a genomic lambda phage library of the homothallic X. parietina. The flanking genes, a homologue of SLA2 of Saccharomyces cerevisiae and a DNA lyase gene, served to amplify the two idiomorphs of the X. polycarpa MAT locus. Each idiomorph contains a single gene: MAT1-2-1 codes for a HMG-box protein, MAT1-1-1 encodes an alpha domain protein. The occurrence of mating-type genes in eight single spore isolates derived from one ascus was studied with a PCR assay. In the homothallic X. parietina a HMG fragment, but no alpha box fragment was found in all isolates, whereas in X. elegans, another homothallic species, all tested isolates contained a fragment of both idiomorphs. Conversely, isolates of the heterothallic X. polycarpa contained either a HMG or an alpha box fragment, but never both.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/280310
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