Back
Full-text
Journal article

On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers.

  • Wojciechowski K Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
  • Orczyk M Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
  • Gutberlet T Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbH, Lichtenbergstr. 1, 85748 Garching, Germany.
  • Brezesinski G Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, Potsdam, Germany.
  • Geue T Laboratory for Neutron Scattering and Imaging, Paul Scherrer Institute , WHGA/110, 5232 Villigen - PSI, Switzerland.
  • Fontaine P Synchrotron SOLEIL, L'Orme des Merisiers, Saint Aubin BP48, Gif-sur-Yvette Cedex, France.
Show more…
  • 2016-08-13
Published in:
  • Langmuir : the ACS journal of surfaces and colloids. - 2016
1,2-Dipalmitoylphosphatidylcholine
Adsorption
Air
Cholesterol
Digitonin
Surface Properties
Thermodynamics
Unilamellar Liposomes
Water
English In this article, we describe the effect of a highly hemolytic saponin, digitonin, on model lipids cholesterol and dipalmitoylphosphatidylcholine (DPPC) using a combination of tensiometric (surface pressure and dilatational surface elasticity), spectroscopic (infrared reflection absorption spectroscopy, IRRAS), microscopic (fluorescence microscopy), and scattering techniques (neutron reflectivity, NR, and grazing incidence X-ray diffraction, GIXD). The monolayers of individual lipids and their 10:9 (mol/mol) mixture were exposed to an aqueous solution of digitonin (10(-4) M) by subphase exchange using a setup developed recently in our laboratory. The results confirm that digitonin can adsorb onto both bare and lipid-covered water-air interfaces. In the case of DPPC, a relatively weak interaction can be observed, but the presence of cholesterol drastically enhances the effect of digitonin. The latter is shown to dissociate the weak cholesterol-DPPC complexes and to bind cholesterol in an additional layer attached to the original lipid monolayer.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/28090
Statistics
Document views: 43 File downloads:
  • Full-text: 0