Blunting the response to endotoxin in healthy subjects: effects of various doses of intravenous fish oil.
- Pittet YK Service of Adult Intensive Care Medicine and Burns Centre, CHUV, 1011 Lausanne, Switzerland.
- Berger MM
- Pluess TT
- Voirol P
- Revelly JP
- Tappy L
- Chioléro RL
- 2009-10-22
Published in:
- Intensive care medicine. - 2010
Adolescent
Adrenocorticotropic Hormone
Adult
Calorimetry, Indirect
Endotoxins
Fish Oils
Humans
Infusions, Intravenous
Male
Prospective Studies
Sepsis
Time Factors
Tumor Necrosis Factor-alpha
Young Adult
English
OBJECTIVE
To test the dose response effect of infused fish oil (FO) rich in n-3 PUFAs on the inflammatory response to endotoxin (LPS) and on membrane incorporation of fatty acids in healthy subjects.
DESIGN
Prospective, sequential investigation comparing three different FO doses.
SUBJECTS
Three groups of male subjects aged 26.8 +/- 3.2 years (BMI 22.5 +/- 2.1).
INTERVENTION
One of three FO doses (Omegaven10%) as a slow infusion before LPS: 0.5 g/kg 1 day before LPS, 0.2 g/kg 1 day before, or 0.2 g/kg 2 h before.
MEASUREMENTS AND RESULTS
Temperature, hemodynamic variables, indirect calorimetry and blood samples (TNF-alpha, stress hormones) were collected. After LPS temperature, ACTH and TNF-alpha concentrations increased in the three groups: the responses were significantly blunted (p < 0.0001) compared with the control group of the Pluess et al. trial. Cortisol was unchanged. Lowest plasma ACTH, TNF-alpha and temperature AUC values were observed after a single 0.2 g/kg dose of FO. EPA incorporation into platelet membranes was dose-dependent.
CONCLUSIONS
Having previously shown that the response to LPS was reproducible, this study shows that three FO doses blunted it to various degrees. The 0.2 g/kg perfusion immediately before LPS was the most efficient in blunting the responses, suggesting LPS capture in addition to the systemic and membrane effects.
To test the dose response effect of infused fish oil (FO) rich in n-3 PUFAs on the inflammatory response to endotoxin (LPS) and on membrane incorporation of fatty acids in healthy subjects.
DESIGN
Prospective, sequential investigation comparing three different FO doses.
SUBJECTS
Three groups of male subjects aged 26.8 +/- 3.2 years (BMI 22.5 +/- 2.1).
INTERVENTION
One of three FO doses (Omegaven10%) as a slow infusion before LPS: 0.5 g/kg 1 day before LPS, 0.2 g/kg 1 day before, or 0.2 g/kg 2 h before.
MEASUREMENTS AND RESULTS
Temperature, hemodynamic variables, indirect calorimetry and blood samples (TNF-alpha, stress hormones) were collected. After LPS temperature, ACTH and TNF-alpha concentrations increased in the three groups: the responses were significantly blunted (p < 0.0001) compared with the control group of the Pluess et al. trial. Cortisol was unchanged. Lowest plasma ACTH, TNF-alpha and temperature AUC values were observed after a single 0.2 g/kg dose of FO. EPA incorporation into platelet membranes was dose-dependent.
CONCLUSIONS
Having previously shown that the response to LPS was reproducible, this study shows that three FO doses blunted it to various degrees. The 0.2 g/kg perfusion immediately before LPS was the most efficient in blunting the responses, suggesting LPS capture in addition to the systemic and membrane effects.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/s00134-009-1689-8
- PMID 19844694
- Persistent URL
- https://sonar.ch/global/documents/281233
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