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Studies of Lassa Virus Cell Entry.
Journal article

Studies of Lassa Virus Cell Entry.

  • Pasquato A Institute of Microbiology, University Hospital Center, University of Lausanne, Rue du Bugnon 48, CH-1011, Lausanne, Switzerland.
  • Fernandez AH Institute of Microbiology, University Hospital Center, University of Lausanne, Rue du Bugnon 48, CH-1011, Lausanne, Switzerland.
  • Kunz S Institute of Microbiology, University Hospital Center, University of Lausanne, Rue du Bugnon 48, CH-1011, Lausanne, Switzerland. Stefan.kunz@chuv.ch.
  • 2017-10-08
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2018
Endocytosis
Endosome
Inhibitor
Lassa virus
Receptor
Viral entry
Animals
Clathrin
Dynamins
Endocytosis
Endosomes
Humans
Lassa virus
Lysosomal-Associated Membrane Protein 1
Protein Binding
Virus Internalization
English Host cell entry is the first and most fundamental step of every virus infection and represents a major barrier for zoonotic transmission and viral emergence. Targeting viral entry appears further as a promising strategy for therapeutic intervention. Several cellular receptors have been identified for Lassa virus, including dystroglycan, TAM receptor tyrosine kinases, and C-type lectins. Upon receptor binding, LASV enters the host cell via a largely unknown clathrin- and dynamin-independent endocytotic pathway that delivers the virus to late endosomes, where fusion occurs after engagement of a second, intracellular receptor, the late endosomal/lysosomal resident protein LAMP1. Here, we describe a series of experimental approaches to investigate LASV cell entry and to test candidate inhibitors for their action at this early and decisive step of infection.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/281349
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