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Live intracellular super-resolution imaging using site-specific stains.
Journal article

Live intracellular super-resolution imaging using site-specific stains.

  • Carlini L Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne , Lausanne, Switzerland.
  • Manley S
  • 2013-10-02
Published in:
  • ACS chemical biology. - 2013
Cell Line, Tumor
Culture Media
Endoplasmic Reticulum
Fluorescent Dyes
Golgi Apparatus
Humans
Lysosomes
Microscopy, Fluorescence
Mitochondria
Molecular Imaging
Staining and Labeling
English Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several live-cell compatible imaging media and evaluated their performance in situ. We identify a number of new dyes and imaging medium-dye combinations for live staining, that densely highlight intracellular structures with excellent photophysical performance for SR.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/282285
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