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Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans.
Journal article

Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans.

  • Koehler M Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium; email: david.alsteens@uclouvain.be.
  • Delguste M Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium; email: david.alsteens@uclouvain.be.
  • Sieben C Institute of Physics, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
  • Gillet L Immunology-Vaccinology Laboratory, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals and Health center (FARAH), University of Liège, 4000 Liège, Belgium.
  • Alsteens D Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium; email: david.alsteens@uclouvain.be.
  • 2020-05-13
Published in:
  • Annual review of virology. - 2020
atomic force microscopy
attachment factors
cell-surface glycans
single-virion level
virus entry
virus-glycan complexes
English Virus infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and cell-surface receptors. Various cell-surface glycans function as initial, usually low-affinity attachment factors, providing a first anchor of the virus to the cell surface, and further facilitate high-affinity binding to virus-specific cell-surface receptors, while other glycans function as specific entry receptors themselves. It is now possible to rapidly identify specific glycan receptors using different techniques, define atomic-level structures of virus-glycan complexes, and study these interactions at the single-virion level. This review provides a detailed overview of the role of glycans in viral infection and highlights experimental approaches to study virus-glycan binding along with specific examples. In particular, we highlight the development of the atomic force microscope to investigate interactions with glycans at the single-virion level directly on living mammalian cells, which offers new perspectives to better understand virus-glycan interactions in physiologically relevant conditions.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/282336
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