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Sample preparation for two-dimensional gel electrophoresis.
Journal article

Sample preparation for two-dimensional gel electrophoresis.

  • Shaw MM Institut de Biologie Cellulaire et de Morphologie, Université de Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland.
  • Riederer BM
  • 2003-08-19
Published in:
  • Proteomics. - 2003
Buffers
Electrophoresis, Gel, Two-Dimensional
Proteins
English The choice of sample preparation protocol is a critical influential factor for isoelectric focusing which in turn affects the two-dimensional gel result in terms of quality and protein species distribution. The optimal protocol varies depending on the nature of the sample for analysis and the properties of the constituent protein species (hydrophobicity, tendency to form aggregates, copy number) intended for resolution. This review explains the standard sample buffer constituents and illustrates a series of protocols for processing diverse samples for two-dimensional gel electrophoresis, including hydrophobic membrane proteins. Current methods for concentrating lower abundance proteins, by removal of high abundance proteins, are also outlined. Finally, since protein staining is becoming increasingly incorporated into the sample preparation procedure, we describe the principles and applications of current (and future) pre-electrophoretic labelling methods.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/282847
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