Journal article

Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.

  • Klein HL Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA.
  • Bačinskaja G Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
  • Che J Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USA.
  • Cheblal A Friedrich Miescher Institute for Biomedical Research (FMI), 4058 Basel, Switzerland.
  • Elango R Department of Biology, University of Iowa, Iowa City, IA, USA.
  • Epshtein A Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA.
  • Fitzgerald DM Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
  • Gómez-González B Centro Andaluz de BIología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla, Seville, Spain.
  • Khan SR Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
  • Kumar S Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Leland BA Yale School of Medicine, New Haven, CT, USA.
  • Marie L Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA.
  • Mei Q Systems, Synthetic and Physical Biology Graduate Program, Rice University, Houston, TX, USA.
  • Miné-Hattab J Institut Curie, PSL Research University, CNRS, UMR3664, F-75005 Paris, France.
  • Piotrowska A NanoBioMedical Centre, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.
  • Polleys EJ Department of Biology, Tufts University, Medford, MA USA.
  • Putnam CD Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, CA, USA.
  • Radchenko EA Department of Biology, Tufts University, Medford, MA USA.
  • Saada AA Institut Curie, PSL Research University, CNRS, UMR3348 F-91405, Orsay, France.
  • Sakofsky CJ Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, Durham, NC, USA.
  • Shim EY Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USA.
  • Stracy M Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
  • Xia J Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
  • Yan Z Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Yin Y Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC USA.
  • Aguilera A Centro Andaluz de BIología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla, Seville, Spain.
  • Argueso JL Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
  • Freudenreich CH Department of Biology, Tufts University, Medford, MA USA.
  • Gasser SM Friedrich Miescher Institute for Biomedical Research (FMI), 4058 Basel, Switzerland.
  • Gordenin DA Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, Durham, NC, USA.
  • Haber JE Department of Biology and Rosenstiel Basic Medical Sciences Research Center Brandeis University, Waltham, MA, USA.
  • Ira G Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Jinks-Robertson S Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC USA.
  • King MC Yale School of Medicine, New Haven, CT, USA.
  • Kolodner RD Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, CA, USA.
  • Kuzminov A Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
  • Lambert SA Institut Curie, PSL Research University, CNRS, UMR3348 F-91405, Orsay, France.
  • Lee SE Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USA.
  • Miller KM Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
  • Mirkin SM Department of Biology, Tufts University, Medford, MA USA.
  • Petes TD Department of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC USA.
  • Rosenberg SM Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
  • Rothstein R Department of Genetics & Development, Columbia University Irving Medical Center, New York, NY, USA.
  • Symington LS Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA.
  • Zawadzki P NanoBioMedical Centre, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.
  • Kim N Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, Houston, TX, USA.
  • Lisby M Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
  • Malkova A Department of Biology, University of Iowa, Iowa City, IA, USA.
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  • 2019-01-18
Published in:
  • Microbial cell (Graz, Austria). - 2019
English Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/283022
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