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Journal article

False-negative PCR result due to gene polymorphism: the example of Neisseria meningitidis.

  • Jaton K Institute of Microbiology, University Hospital of Lausanne (CHUV), Bugnon 48, 1011 Lausanne, Switzerland. Katia.Jaton-Ogay@chuv.ch
  • Ninet B
  • Bille J
  • Greub G
  • 2010-10-22
Published in:
  • Journal of clinical microbiology. - 2010
Bacterial Proteins
Bacteriological Techniques
Child, Preschool
DNA, Bacterial
False Negative Reactions
Humans
Male
Meningococcal Infections
Molecular Sequence Data
Neisseria meningitidis
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Analysis, DNA
English Early treatment of meningococcal meningitis is mandatory but may negate the cerebrospinal fluid culture. Etiological diagnosis then mainly relies on PCR. Here, we report a case of false-negative results for real-time PCR for a Neisseria meningitidis serogroup B isolate with a polymorphism in the ctrA gene.
Language
  • English
Open access status
bronze
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Persistent URL
https://sonar.ch/global/documents/283069
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