Fundus autofluorescence imaging.

Schmitz-Valckenberg S; Pfau M; Fleckenstein M; Staurenghi G; Sparrow JR; Bindewald-Wittich A; Spaide RF; Wolf S; Sadda SR; Holz FG

doi:10.1016/j.preteyeres.2020.100893

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Journal article

Fundus autofluorescence imaging.

Schmitz-Valckenberg S Department of Ophthalmology, University of Bonn, Bonn, Germany; John A. Moran Eye Center, University of Utah, Salt Lake City, USA.
Pfau M Department of Ophthalmology, University of Bonn, Bonn, Germany; Department of Biomedical Data Science, Stanford University, USA.
Fleckenstein M Department of Ophthalmology, University of Bonn, Bonn, Germany.
Staurenghi G Department of Biomedical and Clinical Science "Luigi Sacco", Luigi Sacco Hospital University of Milan, Italy.
Sparrow JR Departments of Ophthalmology and Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA.
Bindewald-Wittich A Department of Ophthalmology, University of Bonn, Bonn, Germany; Augenheilkunde Heidenheim MVZ, Heidenheim, Germany.
Spaide RF Vitreous Retina Macula Consultants of New York, New York, NY, USA.
Wolf S Department of Ophthalmology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Sadda SR Doheny Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, USA.
Holz FG Department of Ophthalmology, University of Bonn, Bonn, Germany. Electronic address: frank.holz@ukbonn.de.

2020-08-08

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Progress in retinal and eye research. - 2020

English Fundus autofluorescence (FAF) imaging is an in vivo imaging method that allows for topographic mapping of naturally or pathologically occurring intrinsic fluorophores of the ocular fundus. The dominant sources are fluorophores accumulating as lipofuscin in lysosomal storage bodies in postmitotic retinal pigment epithelium cells as well as other fluorophores that may occur with disease in the outer retina and subretinal space. Photopigments of the photoreceptor outer segments as well as macular pigment and melanin at the fovea and parafovea may act as filters of the excitation light. FAF imaging has been shown to be useful with regard to understanding of pathophysiological mechanisms, diagnostics, phenotype-genotype correlation, identification of prognostic markers for disease progression, and novel outcome parameters to assess efficacy of interventional strategies in chorio-retinal diseases. More recently, the spectrum of FAF imaging has been expanded with increasing use of green in addition to blue FAF, introduction of spectrally-resolved FAF, near-infrared FAF, quantitative FAF imaging and fluorescence life time imaging (FLIO). This article gives an overview of basic principles, FAF findings in various retinal diseases and an update on recent developments.

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