Journal article
High level of fecal calprotectin at age 2 months as a marker of intestinal inflammation predicts atopic dermatitis and asthma by age 6.
- Orivuori L Institute of Clinical Medicine, University of Helsinki, Helsinki, Finland.
- Mustonen K Environmental Health Department, National Institute for Health and Welfare, Kuopio, Finland.
- de Goffau MC Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
- Hakala S Department of Vaccination and Immune Protection, National Institute for Health and Welfare, Helsinki, Finland.
- Paasela M Department of Vaccination and Immune Protection, National Institute for Health and Welfare, Helsinki, Finland.
- Roduit C Christine Kühne - Center for Allergy Research and Education (CK-CARE), Davos, Switzerland.
- Dalphin JC The Department of Respiratory Disease, Université de Franche-Comté, UMR-CNRS Chrono-Environnement, University Hospital, Besançon, France.
- Genuneit J Institute of Epidemiology and Medical Biometry, Ulm University, Ulm, Germany.
- Lauener R Christine Kühne - Center for Allergy Research and Education (CK-CARE), Davos, Switzerland.
- Riedler J Children and Young Adults' Medicine, Children's Hospital, Schwarzach, Austria.
- Weber J Dr. von Hauner Children's Hospital of the Ludwig Maximilian University Munich, Munich, Germany.
- von Mutius E Dr. von Hauner Children's Hospital of the Ludwig Maximilian University Munich, Munich, Germany.
- Pekkanen J Environmental Health Department, National Institute for Health and Welfare, Kuopio, Finland.
- Harmsen HJM Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
- Vaarala O Institute of Clinical Medicine, University of Helsinki, Helsinki, Finland.
- 2015-03-12
Published in:
- Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. - 2015
Escherichia
Lactobacillaceae
asthma
atopic dermatitis
children
colonization
farming
fecal calprotectin
lipopolysaccharide
Age Factors
Asthma
Bacteria
Biomarkers
Child
Child, Preschool
Cohort Studies
Dermatitis, Atopic
Feces
Female
Gastrointestinal Microbiome
Humans
Hypersensitivity
Infant
Interleukin-10
Intestinal Diseases
Leukocyte L1 Antigen Complex
Leukocytes, Mononuclear
Male
Odds Ratio
Pregnancy
Risk Factors
Surveys and Questionnaires
English
BACKGROUND
Gut microbiota and intestinal inflammation regulate the development of immune-mediated diseases, such as allergies. Fecal calprotectin is a biomarker of intestinal inflammation.
OBJECTIVE
We evaluated the association of early-age fecal calprotectin levels to the later development of allergic diseases in children from farming and non-farming environments and further studied the effect of gut microbiota on the fecal calprotectin levels.
METHODS
Fecal calprotectin was measured from 758 infants participating in the PASTURE study at the age of 2 months using the ELISA method. Serum-specific IgE levels were measured at 6 years of age. Data of environmental factors, doctor-diagnosed atopic dermatitis (AD) and asthma were collected by questionnaire. Multivariate logistic regression models were used for analysis. The composition of fecal microbiota was analysed in a subgroup of 120 infants with 16S rRNA pyrosequencing. The effect of Escherichia coli lipopolysaccharide (LPS) on in vitro monocyte IL-10 secretion was studied by flow cytometry.
RESULTS
The infants with high fecal calprotectin levels at 2 months, that is above the 90th percentile, had an increased risk of developing AD and asthma/asthmatic bronchitis by the age of 6 years (aOR 2.02 (1.06-3.85) and 2.41 (1.25-4.64), respectively). High fecal calprotectin levels correlated negatively with fecal Escherichia. LPS from E. coli stimulated production of IL-10 in monocytes.
CONCLUSION AND CLINICAL RELEVANCE
High degree intestinal inflammation at 2 months of age, detected as high fecal calprotectin, predicted asthma and AD by the age of 6 years and was linked to low abundance of fecal Escherichia. Impaired IL-10 activation due to the lack of colonization with E. coli could explain the intestinal inflammation associated high fecal calprotectin and later risk of asthma and AD. Our results have implications for the design of probiotic treatments and suggest that early intestinal colonization has long-term health effects.
Gut microbiota and intestinal inflammation regulate the development of immune-mediated diseases, such as allergies. Fecal calprotectin is a biomarker of intestinal inflammation.
OBJECTIVE
We evaluated the association of early-age fecal calprotectin levels to the later development of allergic diseases in children from farming and non-farming environments and further studied the effect of gut microbiota on the fecal calprotectin levels.
METHODS
Fecal calprotectin was measured from 758 infants participating in the PASTURE study at the age of 2 months using the ELISA method. Serum-specific IgE levels were measured at 6 years of age. Data of environmental factors, doctor-diagnosed atopic dermatitis (AD) and asthma were collected by questionnaire. Multivariate logistic regression models were used for analysis. The composition of fecal microbiota was analysed in a subgroup of 120 infants with 16S rRNA pyrosequencing. The effect of Escherichia coli lipopolysaccharide (LPS) on in vitro monocyte IL-10 secretion was studied by flow cytometry.
RESULTS
The infants with high fecal calprotectin levels at 2 months, that is above the 90th percentile, had an increased risk of developing AD and asthma/asthmatic bronchitis by the age of 6 years (aOR 2.02 (1.06-3.85) and 2.41 (1.25-4.64), respectively). High fecal calprotectin levels correlated negatively with fecal Escherichia. LPS from E. coli stimulated production of IL-10 in monocytes.
CONCLUSION AND CLINICAL RELEVANCE
High degree intestinal inflammation at 2 months of age, detected as high fecal calprotectin, predicted asthma and AD by the age of 6 years and was linked to low abundance of fecal Escherichia. Impaired IL-10 activation due to the lack of colonization with E. coli could explain the intestinal inflammation associated high fecal calprotectin and later risk of asthma and AD. Our results have implications for the design of probiotic treatments and suggest that early intestinal colonization has long-term health effects.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1111/cea.12522
- PMID 25758537
- Persistent URL
- https://sonar.ch/global/documents/284464
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