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Streptomycetes in micro-cultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format.
Journal article

Streptomycetes in micro-cultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format.

  • 2001-06-02
Published in:
  • Antonie van Leeuwenhoek. - 2000
Bacteriological Techniques
Biomass
Culture Media
Kinetics
Spores, Bacterial
Streptomyces
Time Factors
English Mycelium-forming Streptomyces strains were grown in one milliliter liquid micro-cultures in square deep-well microtiter plates. Growth was evaluated with respect to biomass formation and production of secondary metabolites which were found to be very similar in the micro-cultures, bioreactor, and shake flask cultivations, respectively. Despite repetitive sampling and extensive growth on the walls of the wells, no cross contamination occurred. Furthermore, we successfully employed cold storage at -20 degrees C of spore suspensions (in the 96-well format), directly prepared from cultures grown on agar in the microtitre plate. Cultures were retrieved by replicating aliquots from the frozen spore suspensions.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/284573
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