Journal article
Inflammation reduces osteoblast cytotoxicity induced by diclofenac: An in vitro study.
- Aguirre J From the Department of Anaesthesiology, Intensive Care and Pain Medicine, Balgrist University Hospital Zurich, Zurich (JA, JMB, BR, AB), Department of Anaesthesiology, Clinica Ars Medica, Gravesano (CC), Department of Anaesthesiology, Ospedale Regionale di Bellinzona e Valli, Bellinzona, Switzerland (AS) and Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA (AB).
- Bonvini JM From the Department of Anaesthesiology, Intensive Care and Pain Medicine, Balgrist University Hospital Zurich, Zurich (JA, JMB, BR, AB), Department of Anaesthesiology, Clinica Ars Medica, Gravesano (CC), Department of Anaesthesiology, Ospedale Regionale di Bellinzona e Valli, Bellinzona, Switzerland (AS) and Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA (AB).
- Rupnik B From the Department of Anaesthesiology, Intensive Care and Pain Medicine, Balgrist University Hospital Zurich, Zurich (JA, JMB, BR, AB), Department of Anaesthesiology, Clinica Ars Medica, Gravesano (CC), Department of Anaesthesiology, Ospedale Regionale di Bellinzona e Valli, Bellinzona, Switzerland (AS) and Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA (AB).
- Camponovo C From the Department of Anaesthesiology, Intensive Care and Pain Medicine, Balgrist University Hospital Zurich, Zurich (JA, JMB, BR, AB), Department of Anaesthesiology, Clinica Ars Medica, Gravesano (CC), Department of Anaesthesiology, Ospedale Regionale di Bellinzona e Valli, Bellinzona, Switzerland (AS) and Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA (AB).
- Saporito A From the Department of Anaesthesiology, Intensive Care and Pain Medicine, Balgrist University Hospital Zurich, Zurich (JA, JMB, BR, AB), Department of Anaesthesiology, Clinica Ars Medica, Gravesano (CC), Department of Anaesthesiology, Ospedale Regionale di Bellinzona e Valli, Bellinzona, Switzerland (AS) and Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA (AB).
- Borgeat A From the Department of Anaesthesiology, Intensive Care and Pain Medicine, Balgrist University Hospital Zurich, Zurich (JA, JMB, BR, AB), Department of Anaesthesiology, Clinica Ars Medica, Gravesano (CC), Department of Anaesthesiology, Ospedale Regionale di Bellinzona e Valli, Bellinzona, Switzerland (AS) and Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA (AB).
- 2020-10-30
Published in:
- European journal of anaesthesiology. - 2020
English
BACKGROUND
Diclofenac and other NSAIDs are routinely used in the postoperative period. Their effect on fracture healing remains unclear and controversial.
OBJECTIVE
The primary outcome was to assess the potential cytotoxicity of clinically relevant concentrations of diclofenac on human osteoblasts.
DESIGN
Laboratory in vitro study.
SETTING
Institute of Physiology, Zurich, Center for Integrative Human Physiology, University of Zurich.
MATERIALS
Monolayers of human osteoblasts.
INTERVENTION(S)
Exposure of human osteoblast monolayers to several concentrations of diclofenac, for different periods of time, with and without an artificially induced inflammatory process.
MAIN OUTCOME MEASURES
Cell count, cell viability, cell proliferation and apoptosis.
RESULTS
A concentration-mediated, time and exposure dependent cytotoxic effect of diclofenac-mediated apoptosis was observed. Stimulated inflammatory conditions seemed to reduce toxic effects.
CONCLUSION
Cytotoxic effects of diclofenac are exposure, time and concentration dependent. Simulating aspects of inflammatory conditions seems to increase resistance to diclofenac cytotoxicity, especially in the presence of higher concentration and longer exposure time.
Diclofenac and other NSAIDs are routinely used in the postoperative period. Their effect on fracture healing remains unclear and controversial.
OBJECTIVE
The primary outcome was to assess the potential cytotoxicity of clinically relevant concentrations of diclofenac on human osteoblasts.
DESIGN
Laboratory in vitro study.
SETTING
Institute of Physiology, Zurich, Center for Integrative Human Physiology, University of Zurich.
MATERIALS
Monolayers of human osteoblasts.
INTERVENTION(S)
Exposure of human osteoblast monolayers to several concentrations of diclofenac, for different periods of time, with and without an artificially induced inflammatory process.
MAIN OUTCOME MEASURES
Cell count, cell viability, cell proliferation and apoptosis.
RESULTS
A concentration-mediated, time and exposure dependent cytotoxic effect of diclofenac-mediated apoptosis was observed. Stimulated inflammatory conditions seemed to reduce toxic effects.
CONCLUSION
Cytotoxic effects of diclofenac are exposure, time and concentration dependent. Simulating aspects of inflammatory conditions seems to increase resistance to diclofenac cytotoxicity, especially in the presence of higher concentration and longer exposure time.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1097/EJA.0000000000001363
- PMID 33122572
- Persistent URL
- https://sonar.ch/global/documents/284955
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