Pheophorbide a May Regulate Jasmonate Signaling during Dark-Induced Senescence.
- Aubry S Institute of Plant and Microbial Biology, University of Zürich, 8008 Zürich, Switzerland.
- Fankhauser N Department for Clinical Research, Clinical Trials Unit, University of Bern, 3012 Bern, Switzerland.
- Ovinnikov S Institute of Plant and Microbial Biology, University of Zürich, 8008 Zürich, Switzerland.
- Pružinská A The Australian Research Council Centre of Excellence in Plant Energy Biology, The University of Western Australia, Perth, Western Australia 6009.
- Stirnemann M Institute of Plant and Microbial Biology, University of Zürich, 8008 Zürich, Switzerland.
- Zienkiewicz K Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Göttingen, 37077 Göttingen, Germany.
- Herrfurth C Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Göttingen, 37077 Göttingen, Germany.
- Feussner I Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Göttingen, 37077 Göttingen, Germany.
- Hörtensteiner S Institute of Plant and Microbial Biology, University of Zürich, 8008 Zürich, Switzerland shorten@botinst.uzh.ch.
- 2019-11-23
Published in:
- Plant physiology. - 2020
English
Chlorophyll degradation is one of the most visible signs of leaf senescence. During senescence, chlorophyll is degraded in the multistep pheophorbide a oxygenase (PAO)/phyllobilin pathway. This pathway is tightly regulated at the transcriptional level, allowing coordinated and efficient remobilization of nitrogen toward sink organs. Using a combination of transcriptome and metabolite analyses during dark-induced senescence of Arabidopsis (Arabidopsis thaliana) mutants deficient in key steps of the PAO/phyllobilin pathway, we show an unanticipated role for one of the pathway intermediates, i.e. pheophorbide a Both jasmonic acid-related gene expression and jasmonic acid precursors specifically accumulated in pao1, a mutant deficient in PAO. We propose that pheophorbide a, the last intact porphyrin intermediate of chlorophyll degradation and a unique pathway "bottleneck," has been recruited as a signaling molecule of chloroplast metabolic status. Our work challenges the assumption that chlorophyll breakdown is merely a result of senescence, and proposes that the flux of pheophorbide a through the pathway acts in a feed-forward loop that remodels the nuclear transcriptome and controls the pace of chlorophyll degradation in senescing leaves.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1104/pp.19.01115
- PMID 31753845
- Persistent URL
- https://sonar.ch/global/documents/28637
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