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Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor.
Journal article

Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor.

  • Jenny A Department of Cell Biology, Biozentrum, University of Basel, Switzerland.
  • Keller W
  • 1995-07-25
Published in:
  • Nucleic acids research. - 1995
Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
DNA, Complementary
Fungal Proteins
Humans
Molecular Sequence Data
Molecular Weight
Protein Conformation
Protein Sorting Signals
RNA Precursors
RNA-Binding Proteins
Saccharomyces cerevisiae
Sequence Homology, Amino Acid
mRNA Cleavage and Polyadenylation Factors
English 3'-processing of mRNA precursors depends on several protein factors. One of them, cleavage and polyadenylation specificity factor (CPSF) is required for the cleavage of the mRNA precursor or as well as for the tail elongation reaction. We have obtained complementary DNA encoding the 160 kDa subunit, which had previously been shown to interact with the AAUAAA polyadenylation signal. The cDNAs code for an open reading frame of 1444 amino acids. The translated protein has a calculated molecular weight of 161 kDa and a predicted pl of 6.2. Polyclonal antibodies raised against a bacterially expressed fragment of the cDNA recognise 160 kDa subunit of purified calf thymus CPSF. The sequence contains a possible nuclear localisation signal but none of the known RNA binding motifs. It does, however, show sequence similarities to a UV-damaged DNA binding protein (UVdDb).
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/287907
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