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Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.
Journal article

Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.

  • Santiago J Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.
  • Brandt B Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.
  • Wildhagen M Department of Biosciences, Section for Genetic and Evolutionary Biology, University of Oslo, Oslo, Norway.
  • Hohmann U Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.
  • Hothorn LA Institute of Biostatistics, Leibniz University, Hannover, Germany.
  • Butenko MA Department of Biosciences, Section for Genetic and Evolutionary Biology, University of Oslo, Oslo, Norway.
  • Hothorn M Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.
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  • 2016-04-09
Published in:
  • eLife. - 2016
<i>a. thaliana</i>
biophysics
floral abscission
membrane signaling
peptide hormone
plant biology
plant development
protein complexes
receptor kinase
structural biology
Arabidopsis
Arabidopsis Proteins
Binding Sites
Crystallography, X-Ray
Flowers
Gene Expression Regulation, Plant
Protein Binding
Protein Conformation
Protein Kinases
Protein-Serine-Threonine Kinases
Signal Transduction
English Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/28919
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