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Regulation of the vitellogenin gene B1 promoter after transfer into hepatocytes in primary cultures.
Journal article

Regulation of the vitellogenin gene B1 promoter after transfer into hepatocytes in primary cultures.

  • 1998-09-02
Published in:
  • Molecular and cellular endocrinology. - 1998
Animals
Cell Line
Cells, Cultured
Erythrocytes
Estradiol
Ethinyl Estradiol
Female
Gene Expression Regulation
Humans
Liver
Male
Nucleosomes
Organ Specificity
Promoter Regions, Genetic
Response Elements
Restriction Mapping
Sequence Deletion
Transcription Factors
Transfection
Vitellogenins
Xenopus laevis
English The estrogen-dependent and tissue-specific regulation of the Xenopus laevis vitellogenin gene B1 promoter has been studied by lipid-mediated DNA transfer into Xenopus hepatocytes in primary culture. Hepatocytes achieve an efficient hormonal control of this promoter through a functional interaction between the estrogen responsive elements and a promoter proximal region upstream of the TATA box, which is characterized by a high density of binding sites for the transcription factors CTF/NF-1, C/EBP and HNF3. DNA accessibility to restriction enzymes within the chromosomal copy of the vitellogenin gene B1 promoter shows that the estrogen responsive unit and the promoter proximal region are sensitive to digestion in uninduced and estrogen-induced hepatocytes but not in erythrocyte nuclei. Together, these findings support the notion that chromatin configuration as well as the interplay of promoter elements mediate proper hormone-dependent and tissue-specific expression of the B1 vitellogenin gene.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/290135
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