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BMP2 and TGF-β Cooperate Differently during Synovial-Derived Stem-Cell Chondrogenesis in a Dexamethasone-Dependent Manner.

  • Kovermann NJ AO Research Institute, AO Foundation, 7270 Davos, Switzerland. niko2711@hotmail.com.
  • Basoli V AO Research Institute, AO Foundation, 7270 Davos, Switzerland. Valentina.basoli@aofoundation.org.
  • Della Bella E AO Research Institute, AO Foundation, 7270 Davos, Switzerland. elena.dellabella@aofoundation.org.
  • Alini M AO Research Institute, AO Foundation, 7270 Davos, Switzerland. mauro.alini@aofoundation.org.
  • Lischer C Equine Clinic, Free University of Berlin, 14163 Berlin, Germany. christoph.lischer@fu-berlin.de.
  • Schmal H Department of Orthopaedics and Traumatology, Odense University Hospital, 5000 Odense, Denmark. hschmal@health.sdu.dk.
  • Kubosch EJ Department of Orthopedics and Trauma Surgery, Medical Center-Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, 79085 Freiburg, Germany. johanna.kubosch@uniklinik-freiburg.de.
  • Stoddart MJ AO Research Institute, AO Foundation, 7270 Davos, Switzerland. martin.stoddart@aofoundation.org.
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  • 2019-06-28
Published in:
  • Cells. - 2019
BMP-2
TGF-β
chondrogenesis
dexamethasone
synovial derived stromal cell
Adult
Bone Morphogenetic Protein 2
Chondrogenesis
Collagen Type II
DNA
Dexamethasone
Gene Expression Regulation
Glycosaminoglycans
Humans
Male
Middle Aged
Stem Cells
Synovial Membrane
Transforming Growth Factor beta1
Young Adult
English Recent studies highlighting mesenchymal stem cell (MSC) epigenetic memory suggest that a different differentiation medium may be required depending on the tissue of origin. As synovial-derived stem cells (SDSCs) attract interest we aimed to investigate the influence of TGF-β1, BMP-2 and dexamethasone on SDSC chondrogenesis in vitro. We demonstrate that dexamethasone-free medium led to enhanced chondrogenic differentiation at both the mRNA and matrix level. The greatest COL2A1/COL10A1 ratio was detected in cells exposed to a combination medium containing 10 ng/mL BMP-2 and 1 ng/mL TGF-β1 in the absence of dexamethasone, and this was reflected in the total amount of glycosaminoglycans produced. In summary, dexamethasone-free medium containing BMP-2 and TGF-β1 may be the most suitable when using SDSCs for cartilage tissue regeneration.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/290512
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