Evaluation of MRSA-Screen, a Simple Anti-PBP 2a Slide Latex Agglutination Kit, for Rapid Detection of Methicillin Resistance in Staphylococcus aureus
- Cavassini, Matthias Institut de Microbiologie 1 and
- Wenger, Aline Institut de Microbiologie 1 and
- Jaton, Katia Institut de Microbiologie 1 and
- Blanc, Dominique S. Division Autonome de Médecine Préventive Hospitalière, 2 Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
- Bille, Jacques Institut de Microbiologie 1 and
Published in:
- Journal of Clinical Microbiology. - American Society for Microbiology. - 1999, vol. 37, no. 5, p. 1591-1594
English
The MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was blindly compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, and PCR of themecA gene for the detection of methicillin resistance inStaphylococcus aureus . A total of 120 methicillin-susceptible S. aureus (MSSA) and 80 methicillin-resistant S. aureus (MRSA) isolates, defined by the absence or presence of the mecA gene, respectively, were tested. The MRSA-Screen test, the oxacillin disk diffusion test, and the oxacillin-salt agar screening test showed sensitivities of 100, 61.3, and 82.5% and specificities of 99.2, 96.7, and 98.3%, respectively. We conclude that the MRSA-Screen is a very accurate, reliable, and fast test (15 min) for differentiation of MRSA from MSSA colonies on agar plates.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1128/jcm.37.5.1591-1594.1999
- ISSN 1098-660X
- Persistent URL
- https://sonar.ch/global/documents/293569
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