Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells

Nokhbehsaim, Marjan; Eick, Sigrun; Nogueira, Andressa Vilas Boas; Hoffmann, Per; Herms, Stefan; Fröhlich, Holger; Jepsen, Søren; Jäger, Andreas; Cirelli, Joni Augusto; Deschner, James

doi:10.1155/2013/437123

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Journal article

Stimulation of MMP-1 and CCL2 by NAMPT in PDL Cells

Nokhbehsaim, Marjan Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, Germany
Eick, Sigrun ORCID Department of Periodontology, Laboratory of Oral Microbiology, University of Bern, 3010 Bern, Switzerland
Nogueira, Andressa Vilas Boas ORCID Department of Diagnosis and Surgery, School of Dentistry, SP, UNESP, 14801-903 Araraquara, Brazil
Hoffmann, Per ORCID Division of Medical Genetics, University Hospital Basel and Department of Biomedicine, University of Basel, 4058 Basel, Switzerland
Herms, Stefan ORCID Division of Medical Genetics, University Hospital Basel and Department of Biomedicine, University of Basel, 4058 Basel, Switzerland
Fröhlich, Holger Bonn-Aachen International Center for IT, Algorithmic Bioinformatics, University of Bonn, 53113 Bonn, Germany
Jepsen, Søren Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, 53111 Bonn, Germany
Jäger, Andreas Department of Orthodontics, University of Bonn, 53111 Bonn, Germany
Cirelli, Joni Augusto Department of Diagnosis and Surgery, School of Dentistry, SP, UNESP, 14801-903 Araraquara, Brazil
Deschner, James Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, Germany

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Mediators of Inflammation. - Hindawi Limited. - 2013, vol. 2013, p. 1-12

English Periodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium. Obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), may be a pathomechanistic link between both diseases. The aim of this in vitro study was to examine the regulation of periodontal ligament (PDL) cells by NAMPT and its production under inflammatory and infectious conditions. NAMPT caused a significant upregulation of 9 genes and downregulation of 3 genes, as analyzed by microarray analysis. Eight of these genes could be confirmed by real-time PCR: NAMPT induced a significant upregulation of EGR1, MMP-1, SYT7, ITPKA, CCL2, NTM, IGF2BP3, and NRP1. NAMPT also increased significantly the MMP-1 and CCL2 protein synthesis. NAMPT was significantly induced by interleukin-1βand the periodontal microorganismP. gingivalis. NAMPT may contribute to periodontitis through upregulation of MMP-1 and CCL2 in PDL cells. Increased NAMPT levels, as found in obesity, may therefore represent a mechanism whereby obesity could confer an increased risk of periodontitis. Furthermore, microbial and inflammatory signals may enhance the NAMPT synthesis in PDL cells and thereby contribute to the increased gingival and serum levels of this adipokine, as found in periodontitis.

Language

English

Open access status

gold

Identifiers

DOI 10.1155/2013/437123
ISSN 0962-9351

Persistent URL

https://sonar.ch/global/documents/295990

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