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Intrathymic Epstein-Barr virus infection is not a prominent feature of myasthenia gravis.
Journal article

Intrathymic Epstein-Barr virus infection is not a prominent feature of myasthenia gravis.

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  • 2011-09-10
Published in:
  • Annals of neurology. - 2011
Adolescent
Adrenal Cortex Hormones
Adult
Age of Onset
Antigens, CD19
B-Lymphocytes
Cell Lineage
Cytokines
DNA, Viral
Enzyme-Linked Immunosorbent Assay
Epstein-Barr Virus Infections
Female
Flow Cytometry
Humans
Hyperplasia
Immunohistochemistry
Male
Myasthenia Gravis
Plasmids
Receptors, Cholinergic
Reverse Transcriptase Polymerase Chain Reaction
Thymectomy
Thymus Gland
Tumor Necrosis Factor Receptor Superfamily, Member 7
Young Adult
English Lymph node-type T- and B-cell infiltrates with germinal centers are characteristic features of the hyperplastic thymus in early onset myasthenia gravis (EOMG).Epstein-Barr virus (EBV) infection confers survival advantages on B cells, and has recently been implicated in tertiary lymphoid tissue formation in EOMG. We evaluated the frequency of intrathymic EBV-infected B-lineage cells and antiviral immune responses in treatment-naive patients with EOMG. Real-time polymerase chain reaction was performed to quantify the content of genomic EBV DNA (BamHI-W repeat region) in thymic cell suspensions. Serial paraffin sections of EOMG thymi were analyzed for the presence of EBV-encoded RNA by in situ hybridization and for viral gene expression by immunohistochemistry. Humoral and cellular immune responses to viral antigens were quantified by enzyme-linked immunosorbent assay and flow cytometry-based intracellular cytokine staining. We detected minimal levels of viral DNA-corresponding to single viral genomes-in only 6 of 16 hyperplastic EOMG thymi, indicating extreme rarity of viral copy numbers in the investigated thymic samples. That was confirmed by similar rarity of EBV-encoded RNA and viral proteins identified in thymic sections. Furthermore, EBV-specific T- and B-cell responses were unchanged in patients with EOMG. These findings do not support an etiologic role for EBV in the initiation of EOMG.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/298137
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