Back
Analysis of Tooth Innervation in Microfluidic Coculture Devices.
Journal article

Analysis of Tooth Innervation in Microfluidic Coculture Devices.

  • Pagella P Unit of Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland.
  • Mitsiadis TA Unit of Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland. thimios.mitsiadis@zzm.uzh.ch.
  • 2020-06-01
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2020
Coculture systems
Developmental biology
Innervation
Microfluidics
Orofacial development
Tooth
Trigeminal ganglion
English Innervation plays a key role in the development, homeostasis, and regeneration of organs and tissues. However, the mechanisms underlying these phenomena are not well understood yet. In particular, the role of innervation in tooth development and regeneration is neglected. Cocultures constitute a valuable method to investigate and manipulate the interactions between nerve fibers and teeth in a controlled and isolated environment. Microfluidic systems for allow cocultures of neurons and different cell types in their appropriate culture media, while permitting the passage of axons from one compartment to the other. Here we describe how to isolate and coculture developing trigeminal ganglia and tooth germs in a microfluidic coculture system. This protocol describes a simple and flexible way to coculture ganglia/nerves and their target tissues and to study the roles of specific molecules on such interactions in a controlled and isolated environment.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/298706
Statistics
Document views: 20 File downloads: