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Fibroblast growth factor signaling affects vascular outgrowth and is required for the maintenance of blood vessel integrity.
Journal article

Fibroblast growth factor signaling affects vascular outgrowth and is required for the maintenance of blood vessel integrity.

  • De Smet F Laboratory of Angiogenesis & Neurovascular Link, Vesalius Research Center (VRC), VIB, 3000 Leuven, Belgium; Laboratory of Angiogenesis & Neurovascular Link, Department of Oncology, KU Leuven, 3000 Leuven, Belgium.
  • Tembuyser B Laboratory of Angiogenesis & Neurovascular Link, Vesalius Research Center (VRC), VIB, 3000 Leuven, Belgium; Laboratory of Angiogenesis & Neurovascular Link, Department of Oncology, KU Leuven, 3000 Leuven, Belgium.
  • Lenard A Biozentrum der Universität Basel, Klingelbergstrasse 50/70, 4056 Basel, Switzerland.
  • Claes F Laboratory of Angiogenesis & Neurovascular Link, Vesalius Research Center (VRC), VIB, 3000 Leuven, Belgium; Laboratory of Angiogenesis & Neurovascular Link, Department of Oncology, KU Leuven, 3000 Leuven, Belgium.
  • Zhang J Laboratory of Pharmaceutical Analysis, Pharmaceutical Sciences, KU Leuven, 3000 Leuven, Belgium.
  • Michielsen C Laboratory of Pharmaceutical Analysis, Pharmaceutical Sciences, KU Leuven, 3000 Leuven, Belgium.
  • Van Schepdael A Laboratory of Pharmaceutical Analysis, Pharmaceutical Sciences, KU Leuven, 3000 Leuven, Belgium.
  • Herbert JM Early to Candidate Department and Lead Generation and Candidate Realization Department, Sanofi, 31036 Toulouse, France.
  • Bono F Early to Candidate Department and Lead Generation and Candidate Realization Department, Sanofi, 31036 Toulouse, France.
  • Affolter M Biozentrum der Universität Basel, Klingelbergstrasse 50/70, 4056 Basel, Switzerland.
  • Dewerchin M Laboratory of Angiogenesis & Neurovascular Link, Vesalius Research Center (VRC), VIB, 3000 Leuven, Belgium; Laboratory of Angiogenesis & Neurovascular Link, Department of Oncology, KU Leuven, 3000 Leuven, Belgium.
  • Carmeliet P Laboratory of Angiogenesis & Neurovascular Link, Vesalius Research Center (VRC), VIB, 3000 Leuven, Belgium; Laboratory of Angiogenesis & Neurovascular Link, Department of Oncology, KU Leuven, 3000 Leuven, Belgium. Electronic address: peter.carmeliet@vib-kuleuven.be.
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  • 2014-09-10
Published in:
  • Chemistry & biology. - 2014
Animal Fins
Animals
Blood Vessels
Cadherins
Embryo, Nonmammalian
Indolizines
Intercellular Junctions
Neovascularization, Physiologic
Receptors, Fibroblast Growth Factor
Regeneration
Signal Transduction
Zebrafish
Zebrafish Proteins
ortho-Aminobenzoates
English Angiogenesis contributes to the development of numerous disorders. Even though fibroblast growth factors (FGFs) were discovered as mediators of angiogenesis more than 30 years ago, their role in developmental angiogenesis still remains elusive. We use a recently described chemical probe, SSR128129E (SSR), that selectively inhibits the action of multiple FGF receptors (FGFRs), in combination with the zebrafish model to examine the role of FGF signaling in vascular development. We observe that while FGFR signaling is less important for vessel guidance, it affects vascular outgrowth and is especially required for the maintenance of blood vessel integrity by ensuring proper cell-cell junctions between endothelial cells. In conclusion, our work illustrates the power of a small molecule probe to reveal insights into blood vessel formation and stabilization and thus of broad interest to the vascular biology community.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/298962
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