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Site 1 Protease Is Required for Proteolytic Processing of the Glycoproteins of the South American Hemorrhagic Fever Viruses Junin, Machupo, and Guanarito

  • Rojek, Jillian M. Molecular and Integrative Neurosciences Department (MIND), The Scripps Research Institute, La Jolla, California 92037
  • Lee, Andrew M. Molecular and Integrative Neurosciences Department (MIND), The Scripps Research Institute, La Jolla, California 92037
  • Nguyen, NgocThao Molecular and Integrative Neurosciences Department (MIND), The Scripps Research Institute, La Jolla, California 92037
  • Spiropoulou, Christina F. Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
  • Kunz, Stefan Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland
Published in:
  • Journal of Virology. - American Society for Microbiology. - 2008, vol. 82, no. 12, p. 6045-6051
Immunology
Insect Science
Microbiology
Virology
English ABSTRACT
The cellular proprotein convertase site 1 protease (S1P) has been implicated in the proteolytic processing of the glycoproteins (GPs) of Old World arenaviruses. Here we report that S1P is also involved in the processing of the GPs of the genetically more-distant South American hemorrhagic fever viruses Guanarito, Machupo, and Junin. Efficient cleavage of Guanarito virus GP, whose protease recognition sites deviate from the reported S1P consensus sequence, indicates a broader specificity of S1P than anticipated. Lack of GP processing of Junin virus dramatically reduced production of infectious virus and prevented cell-to-cell propagation. Infection of S1P-deficient cells resulted in viral persistence over several weeks without the emergence of escape variants able to use other cellular proteases for GP processing.
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  • English
Open access status
bronze
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https://sonar.ch/global/documents/29937
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