Visual histological grading system for the evaluation of in vitro-generated neocartilage.
- Grogan SP Osteoarticular Research Group, Institute of Pathology, University of Bern, Bern, Switzerland.
- Barbero A
- Winkelmann V
- Rieser F
- Fitzsimmons JS
- O'Driscoll S
- Martin I
- Mainil-Varlet P
- 2006-09-14
Published in:
- Tissue engineering. - 2006
English
Here we present the development of a visual evaluation system for routine assessment of in vitro-engineered cartilaginous tissue. Neocartilage was produced by culturing human articular chondrocytes in pellet culture systems or in a scaffold-free bioreactor system. All engineered tissues were embedded in paraffin and were sectioned and stained with Safranin O-fast green. The evaluation of each sample was broken into 3 categories (uniformity and intensity of Safranin O stain, distance between cells/amount of matrix produced, and cell morphology), and each category had 4 components with a score ranging from 0 to 3. Three observers evaluated each sample, and the new system was independently tested against an objective computer-based histomorphometry system. Pellets were also assessed biochemically for glycosaminoglycan (GAG) content. Pellet histology scores correlated significantly with GAG contents and were in agreement with the computer-based histomorphometry system. This system allows a valid and rapid assessment of in vitro-generated cartilaginous tissue that has a relevant association with objective parameters indicative of cartilage quality.
- Language
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- English
- Open access status
- green
- Identifiers
-
- DOI 10.1089/ten.2006.12.2141
- PMID 16968155
- Persistent URL
- https://sonar.ch/global/documents/306
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