Identification of phytotoxins from Botryosphaeria obtusa , a pathogen of black dead arm disease of grapevine
csal
Djoukeng, JulesInstitute of Biology, University of Neuchâtel, rue Emile Argand 11, 2009, Neuchâtel, Switzerland
Polli, SuzannaInstitute of Biology, University of Neuchâtel, rue Emile Argand 11, 2009, Neuchâtel, Switzerland
Larignon, PhilippeInstitut Français de la Vigne et du Vin, Unité Rhône-Méditerranée, Domaine de Donadille, 30230, Rodilhan, France
Abou-Mansour, ElianeInstitute of Biology, University of Neuchâtel, rue Emile Argand 11, 2009, Neuchâtel, Switzerland
2009
Published in:
European Journal of Plant Pathology. - Springer Netherlands. - 2009, vol. 124, no. 2, p. 303-308
English
A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7-hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ -resveratrol into the dimer delta-viniferin. The structures of isolated phytotoxins were established on the basis of IR, MS, 1D and 2D NMR