Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Lescat, Mathilde; Poirel, Laurent; Nordmann, Patrice

doi:10.1016/j.diagmicrobio.2018.04.010

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Journal article

Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Université de Fribourg

Lescat, Mathilde INSERM European Unit (LEA, IAME), Paris, France - Université Paris Nord, Sorbonne Paris Cité, Paris, France - AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Bondy, France
Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris, France - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA, IAME), Paris, France - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland

01.12.2018

Published in:

Diagnostic Microbiology and Infectious Disease. - 2018, vol. 92, no. 4, p. 267–269

French A rapid (total time <2 h) and reliable multiplex polymerase chain reaction for screening of mcr-1 to mcr-5 genes conferring resistance to colistin has been developed. This technique has been tested on a collection of isolates previously identified as bearing mcr-1, mcr-2, and mcr-like genes and had a sensitivity and a specificity of 100%. Using this method, we were also able to identify a single isolate possessing both mcr- 1 and mcr-5 genes.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language