Strain Fidelity of Chronic Wasting Disease upon Murine Adaptation
- Sigurdson, Christina J. University Hospital Zurich, Institute of Neuropathology, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
- Manco, Giuseppe University Hospital Zurich, Institute of Neuropathology, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
- Schwarz, Petra University Hospital Zurich, Institute of Neuropathology, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
- Liberski, Pawel Department of Molecular Pathology, Medical University Lodz, Czechoslowacka Street 8/10, Pl 92-216 Lodz, Poland
- Hoover, Edward A. Department of Microbiology, Immunology, and Pathology, Colorado State University, Campus Delivery 1619, Fort Collins, Colorado, 80523-1619
- Hornemann, Simone Institute of Molecular Biology and Biophysics, HPK G4, ETH Zurich, CH-8093 Zürich, Switzerland
- Polymenidou, Magdalini University Hospital Zurich, Institute of Neuropathology, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
- Miller, Michael W. Colorado Division of Wildlife, Wildlife Research Center, 317 West Prospect Road, Fort Collins, Colorado 80526-2097
- Glatzel, Markus University Hospital Zurich, Institute of Neuropathology, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
- Aguzzi, Adriano University Hospital Zurich, Institute of Neuropathology, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
Published in:
- Journal of Virology. - American Society for Microbiology. - 2006, vol. 80, no. 24, p. 12303-12311
English
ABSTRACT
Chronic wasting disease (CWD), a prion disease of deer and elk, is highly prevalent in some regions of North America. The establishment of mouse-adapted CWD prions has proven difficult due to the strong species barrier between mice and deer. Here we report the efficient transmission of CWD to transgenic mice overexpressing murine PrP. All mice developed disease 500 ± 62 days after intracerebral CWD challenge. The incubation period decreased to 228 ± 103 days on secondary passage and to 162 ± 6 days on tertiary passage. Mice developed very large, radially structured cerebral amyloid plaques similar to those of CWD-infected deer and elk. PrPSc was detected in spleen, indicating that murine CWD was lymphotropic. PrPSc glycoform profiles maintained a predominantly diglycosylated PrP pattern, as seen with CWD in deer and elk, across all passages. Therefore, all pathological, biochemical, and histological strain characteristics of CWD appear to persist upon repetitive serial passage through mice. These findings indicate that the salient strain-specific properties of CWD are encoded by agent-intrinsic components rather than by host factors.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1128/jvi.01120-06
- ISSN 0022-538X
- Persistent URL
- https://sonar.ch/global/documents/30955
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