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Stable human lymphoblastoid cell lines constitutively expressing hepatitis C virus proteins.
Journal article

Stable human lymphoblastoid cell lines constitutively expressing hepatitis C virus proteins.

  • Wölk B Department of Medicine II, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany.
  • Gremion C Clinic for Rheumatology and Clinical Immunology/Allergology, Inselspital, University of Bern, CH-3010 Bern, Switzerland.
  • Ivashkina N Department of Medicine II, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany.
  • Engler OB Clinic for Rheumatology and Clinical Immunology/Allergology, Inselspital, University of Bern, CH-3010 Bern, Switzerland.
  • Grabscheid B Clinic for Rheumatology and Clinical Immunology/Allergology, Inselspital, University of Bern, CH-3010 Bern, Switzerland.
  • Bieck E Department of Medicine II, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany.
  • Blum HE Department of Medicine II, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany.
  • Cerny A Department of Medicine, Ospedale Regionale di Lugano, Via Tesserete 46, CH-6903 Lugano, Switzerland.
  • Moradpour D Department of Medicine II, University of Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany.
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  • 2005-05-26
Published in:
  • The Journal of general virology. - 2005
Animals
Cell Line, Tumor
Clone Cells
Cytoplasm
HLA-A2 Antigen
Hepacivirus
Hepatitis C
Mice
Mice, Transgenic
Polyproteins
Viral Hepatitis Vaccines
Viral Nonstructural Proteins
Viral Structural Proteins
English The cellular immune response plays a central role in virus clearance and pathogenesis of liver disease in hepatitis C. The study of hepatitis C virus (HCV)-specific immune responses is limited by currently available cell-culture systems. Here, the establishment and characterization of stable human HLA-A2-positive B-lymphoblastoid x T hybrid cell lines constitutively expressing either the NS3-4A complex or the entire HCV polyprotein are reported. These cell lines, termed T1/NS3-4A and T1/HCVcon, respectively, were maintained in continuous culture for more than 1 year with stable characteristics. HCV structural and non-structural proteins were processed accurately, indicating that the cellular and viral proteolytic machineries are functional in these cell lines. Viral proteins were found in the cytoplasm in dot-like structures when expressed in the context of the HCV polyprotein or in a perinuclear fringe when the NS3-4A complex was expressed alone. T1/NS3-4A and T1/HCVcon cells were lysed efficiently by HCV-specific cytotoxic T lymphocytes from patients with hepatitis C and from human HLA-A2.1 transgenic mice immunized with a liposomal HCV vaccine, indicating that viral proteins are processed endogenously and presented efficiently via the major histocompatibility complex class I pathway. In conclusion, these cell lines represent a unique tool to study the cellular immune response, as well as to evaluate novel vaccine and immunotherapeutic strategies against HCV.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.ch/global/documents/316
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