Stability engineering of antibody single-chain Fv fragments.
- Wörn A Biochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057, Switzerland.
- Plückthun A
- 2001-02-13
Published in:
- Journal of molecular biology. - 2001
Animals
Binding Sites
Complementarity Determining Regions
Directed Molecular Evolution
Disulfides
Humans
Immunoglobulin Variable Region
Mutation
Peptide Library
Protein Engineering
Protein Structure, Tertiary
Thermodynamics
English
The application of single-chain Fv fragments (scFv) in medicine and biotechnology places great demands on their stability. Only recently has attention been given to the production of highly stable scFvs, and in a number of examples it was found that such fragments indeed perform better during practical applications. The structural parameters influencing scFv stability are now beginning to be elucidated. This review summarizes progress in rational and evolutionary engineering methods, the structural implications of these results, as well as some examples where stability engineering has been successfully applied.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1006/jmbi.2000.4265
- PMID 11162109
- Persistent URL
- https://sonar.ch/global/documents/31974
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