A simple quantitative culture of Malassezia spp. in HIV-positive persons.
- Pechère M Department of Dermatology, Geneva University Hospital, Switzerland.
- Remondat C
- Bertrand C
- Didierjean L
- Saurat JH
- 1995-01-01
Published in:
- Dermatology (Basel, Switzerland). - 1995
Colony Count, Microbial
Culture Media
Forehead
HIV Seronegativity
HIV Seropositivity
Humans
Malassezia
Mycology
Reproducibility of Results
Skin
English
BACKGROUND
Etiological role of Malassezia spp. remains controversial in certain skin diseases.
OBJECTIVE
To adapt a 'tape method' for quantitative culture of Malassezia spp.
METHOD
Samples for culture were taken from clinically normal forehead skin of HIV-positive and negative persons by stripping with a tape that was then placed on Leeming & Notman medium. The number of colonies was counted after 14 days.
RESULTS
74/78 (94.8%) cultures were positive, for a median count of 9 CFU/tape (range 0 to > 200). High skin density of Malassezia spp., defined as more than 100 CFU/tape, was found in 7/38 (18.4%) HIV-positive persons and was absent (0/40) in the HIV-negative group (p < 0.01).
CONCLUSION
The method used is simple, unexpensive and reliable. High Malassezia spp. density was only found in HIV-positive patients.
Etiological role of Malassezia spp. remains controversial in certain skin diseases.
OBJECTIVE
To adapt a 'tape method' for quantitative culture of Malassezia spp.
METHOD
Samples for culture were taken from clinically normal forehead skin of HIV-positive and negative persons by stripping with a tape that was then placed on Leeming & Notman medium. The number of colonies was counted after 14 days.
RESULTS
74/78 (94.8%) cultures were positive, for a median count of 9 CFU/tape (range 0 to > 200). High skin density of Malassezia spp., defined as more than 100 CFU/tape, was found in 7/38 (18.4%) HIV-positive persons and was absent (0/40) in the HIV-negative group (p < 0.01).
CONCLUSION
The method used is simple, unexpensive and reliable. High Malassezia spp. density was only found in HIV-positive patients.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1159/000246598
- PMID 8573942
- Persistent URL
- https://sonar.ch/global/documents/33137
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